Overview

  • Product name

  • Description

    Rabbit polyclonal to AKR7A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 84 - 357 of Human AKR7A2 (NP_003680).

  • Positive control

    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
ICC/IF 1/100 - 1/200.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
  • Tissue specificity

    Detected in brain, liver, small intestine and testis, and at lower levels in heart, prostate, skeletal muscle and spleen. Detected in kidney proximal and distal tubules, endothelial cells lining the Bowman's capsules and some cysts. Detected at low levels in lung and pancreas (at protein level). Widely expressed.
  • Sequence similarities

    Belongs to the aldo/keto reductase 2 family.
  • Cellular localization

    Golgi apparatus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AFAR antibody
    • AFAR1 antibody
    • AFB1 aldehyde reductase 1 antibody
    • AFB1 AR1 antibody
    • AFB1-AR 1 antibody
    • AFB1AR1 antibody
    • Aflatoxin aldehyde reductase antibody
    • Aflatoxin B1 aldehyde reductase member 2 antibody
    • Aflatoxin beta1 aldehyde reductase antibody
    • Aiar antibody
    • AKR7 antibody
    • Akr7a2 antibody
    • Aldo keto reductase family 7 antibody
    • Aldo keto reductase family 7 member A2 aflatoxin aldehyde reductase antibody
    • Aldo keto reductase family 7 member A2 antibody
    • Aldo keto reductase family 7, member A2 (aflatoxin aldehyde reductase) antibody
    • Aldoketoreductase 7 antibody
    • ARK72_HUMAN antibody
    • SSA reductase antibody
    • Succinic semialdehyde reductase antibody
    see all

Images

  • Anti-AKR7A2 antibody (ab97458) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • ab97458, at a 1/200 dilution, staining AKR7A2 in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
  • Immunohistochemical analysis of PFA fixed, paraffin-embedded mouse fore brain tissue labeling AKR7A2 with a97458 at 1/500 dilution.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Tissue lysate - whole (Heart)
Loading amount
50 µg
Specification
Heart
Gel Running Conditions
Non-reduced Denaturing (12% Tris/glycine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Nov 16 2012

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