Product nameAnti-AKT (phospho T308) antibody
DescriptionRabbit polyclonal to AKT (phospho T308)
Specificityab38449 recognises endogenous levels of AKT (pT308), AKT2 (pT309) and AKT3 (pT305) only when phosphorylated at those residues.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human AKT aa 276-325.
Database link: P31749
- WB: HeLa treated with Insulin. IHC-P: human lung carcinoma tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab38449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 56 kDa.
Block with BSA.
|IHC-P||Use at an assay dependent concentration.|
RelevanceAKT, also known as protein kinase B (PKB), is a serine/threonine protein kinase. There are three mammalian isoforms of AKT: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphoylate and inactivate several targets, including Bad, Forkhead transcription factors, and caspase 9. Activity of AKT has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to AKT activity, however phosphorylation of T308 has been shown to be absolutely essential for AKT activation.
Cellular localizationCell Membrane, Cytoplasmic and Nuclear. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
- AKT1 antibody
- C AKT antibody
- PKB antibody
All lanes : Anti-AKT (phospho T308) antibody (ab38449) at 1/1000 dilution
Lane 1 : HeLa cells
Lane 2 : HeLa cells treated with 0.01 U/mL Insulin for 15 minutes
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 56 kDa
Running conditions: 60v, 30min; 120v 60min
Transfer conditions: 150mA 120min Nitrocellulose membrane.
Blocking conditions: 5% non-fat milk in TBST, RT, 90min.
Primary antibody incubation: 4?, overnight
Secondary antibody incubation: room temperature for 45 minutes
Washing condition: 5 ml TBST, 4 x 5min
Immunohistochemical analysis of AKT (phospho T308) expression in paraffin embedded human lung carcinoma tissue, using ab38449 (1/50). Right-hand panel represents a negative control where ab38449 was pre-incubated with the immunizing (blocking) peptide.
All lanes : Anti-AKT (phospho T308) antibody (ab38449) at 1/500 dilution
Lane 1 : 293 cell lysate - untreated
Lane 2 : 293 cell lysate - treated with insulin
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
This product has been referenced in:
- Dong L et al. LINC00461 promotes cell migration and invasion in breast cancer through miR-30a-5p/integrin ß3 axis. J Cell Biochem 120:4851-4862 (2019). Read more (PubMed: 30623482) »
- Yuan YH et al. Epigenetic inactivation of HOXD10 is associated with human colon cancer via inhibiting the RHOC/AKT/MAPK signaling pathway. Cell Commun Signal 17:9 (2019). Read more (PubMed: 30683109) »