Overview

  • Product name

    Anti-AKT1 + AKT2 + AKT3 antibody
    See all AKT1 + AKT2 + AKT3 primary antibodies
  • Description

    Rabbit polyclonal to AKT1 + AKT2 + AKT3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human
    Predicted to work with: Sheep, Goat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human AKT1 + AKT2 + AKT3 aa 466-480 (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    CRPHFPQFSYSASGTA


    (Peptide available as ab9041)

  • Positive control

    • Human colon tumour tissue, Human liver tissue, Cultured neonatal rat cardiomyocytes expressing an AKT construct

Properties

Applications

Our Abpromise guarantee covers the use of ab106693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent concentration.
ELISA 1/2000 - 1/10000.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
  • Tissue specificity

    In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Domain

    Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
  • Post-translational
    modifications

    Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Cellular localization

    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • Database links

  • Alternative names

    • AKT antibody
    • AKT1 antibody
    • AKT1 kinase antibody
    • AKT1m antibody
    • AKT2 antibody
    • AKT2 kinase antibody
    • Akt3 antibody
    • AKT3_HUMAN antibody
    • CAKT antibody
    • CWS6 antibody
    • DKFZp434N0250 antibody
    • HIHGHH antibody
    • kinase Akt1 antibody
    • MGC99656 antibody
    • MPPH antibody
    • Murine thymoma viral (v-akt) homolog 2 antibody
    • pan-akt antibody
    • PKB ALPHA antibody
    • PKB antibody
    • PKB beta antibody
    • PKB gamma antibody
    • PKB-GAMMA antibody
    • PKB/Akt antibody
    • PKBALPHA antibody
    • PKBB antibody
    • PKBBETA antibody
    • PKBG antibody
    • PKBGAMMA antibody
    • PRKBA antibody
    • PRKBB antibody
    • PRKBG antibody
    • Protein kinase Akt 2 antibody
    • Protein kinase Akt-2 antibody
    • Protein kinase Akt-3 antibody
    • Protein kinase B alpha antibody
    • Protein kinase B antibody
    • Protein kinase B beta antibody
    • Protein kinase B gamma antibody
    • Proto oncogene c Akt antibody
    • Proto-oncogene c-Akt antibody
    • RAC ALPHA antibody
    • RAC alpha serine/threonine protein kinase antibody
    • RAC antibody
    • RAC BETA antibody
    • RAC beta serine/threonine protein kinase antibody
    • RAC PK alpha antibody
    • RAC PK beta antibody
    • rac protein kinase alpha antibody
    • rac protein kinase beta antibody
    • RAC-ALPHA antibody
    • RAC-alpha serine/threonine-protein kinase antibody
    • RAC-beta serine/threonine-protein kinase antibody
    • RAC-gamma antibody
    • RAC-gamma serine/threonine-protein kinase antibody
    • RAC-PK-alpha antibody
    • RAC-PK-beta antibody
    • RAC-PK-gamma antibody
    • RACALPHA antibody
    • RACalpha serine/threonine kinase antibody
    • RACBETA antibody
    • RACgamma antibody
    • RACgamma serine/threonine protein kinase antibody
    • RACPKgamma antibody
    • serine threonine protein kinase antibody
    • STK 2 antibody
    • STK-2 antibody
    • STK2 antibody
    • thymoma viral proto oncogene 1 antibody
    • thymoma viral proto oncogene antibody
    • V akt murine thymoma viral oncogene homolog 1 antibody
    • V akt murine thymoma viral oncogene homolog 2 antibody
    • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
    • V akt murine thymoma viral oncogene homolog 3 antibody
    • V-AKT murine thymoma viral oncogene homolog 1 antibody
    • V-AKT murine thymoma viral oncogene homolog 2 antibody
    • V-AKT murine thymoma viral oncogene homolog 3 antibody
    • vakt murine thymoma viral oncogene homolog 1 antibody
    • vakt murine thymoma viral oncogene homolog 2 antibody
    • vakt murine thymoma viral oncogene homolog 3 antibody
    see all

Images

  • ab106693, at 1/100 dilution, staing AKT1 + AKT2 + AKT3 in Human liver followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • ab106693 at 1/100 dilution, staining AKT1 + AKT2 + AKT3 in Human colon, A) normal tissue B) tumour tissue.

  • ab106693, at 1/80 dilution, staining AKT1 + AKT2 + AKT3 in cultured neonatal Rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT1/2/3 staining appears green. Actin filaments are labeled red using a Texas-red™ conjugated phalloidin.

References

This product has been referenced in:

  • Wang C  et al. Investigation of the neuroprotective effects of crocin via antioxidant activities in HT22 cells and in mice with Alzheimer's disease. Int J Mol Med 43:956-966 (2019). Read more (PubMed: 30569175) »
  • Zhou P  et al. Protective Effects of Total Saponins of Aralia elata (Miq.) on Endothelial Cell Injury Induced by TNF-a via Modulation of the PI3K/Akt and NF-?B Signalling Pathways. Int J Mol Sci 20:N/A (2018). Read more (PubMed: 30577658) »
See all 6 Publications for this product

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