Product nameAnti-AKT1 + AKT2 + AKT3 antibody
See all AKT1 + AKT2 + AKT3 primary antibodies
DescriptionRabbit polyclonal to AKT1 + AKT2 + AKT3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human AKT1 + AKT2 + AKT3 aa 416-480. The exact sequence is proprietary.
- 293T, A431, HeLa, HepG2 and A375 whole cell lysate/extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 78% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab184136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 56 kDa.|
FunctionIGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
Tissue specificityIn adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
modificationsPhosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
- Information by UniProt
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
All lanes : Anti-AKT1 + AKT2 + AKT3 antibody (ab184136) at 1/1000 dilution
Lane 1 : 293T whole cell lysate/extract
Lane 2 : A431 whole cell lysate/extract
Lane 3 : HeLa whole cell lysate/extract
Lane 4 : HepG2 whole cell lysate/extract
Lane 5 : A375 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
10 % SDS-PAGE