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    akt1--akt2--akt3-antibody-ab184136.pdf

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Neuroscience Neurotransmission Intracellular Signaling Kinases
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Anti-AKT1 + AKT2 + AKT3 antibody (ab184136)

  • Datasheet
  • SDS
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Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab184136)

    Key features and details

    • Rabbit polyclonal to AKT1 + AKT2 + AKT3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-AKT1 + AKT2 + AKT3 antibody
      See all AKT1 + AKT2 + AKT3 primary antibodies
    • Description

      Rabbit polyclonal to AKT1 + AKT2 + AKT3
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human AKT1 + AKT2 + AKT3 aa 416-480. The exact sequence is proprietary.

    • Positive control

      • 293T, A431, HeLa, HepG2 and A375 whole cell lysate/extracts.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Intracellular Signaling
      • Kinases
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • PKB / AKT
      • Signal Transduction
      • Growth Factors/Hormones
      • Insulin / Insulin-like
      • Cancer
      • Signal transduction
      • Protein phosphorylation
      • Serine/threonine kinases
      • AKT
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Transcription factors
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Cell survival & death

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HepG2 whole cell lysate (ab166833)
      • HeLa whole cell lysate (ab29545)
      • A431 whole cell lysate (ab7909)

    Applications

    Our Abpromise guarantee covers the use of ab184136 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.

    Target

    • Function

      IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
    • Tissue specificity

      In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • Domain

      Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
    • Post-translational
      modifications

      Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
    • Cellular localization

      Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
    • Target information above from: UniProt accession Q9Y243 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10000 Human
      • Entrez Gene: 207 Human
      • Entrez Gene: 208 Human
      • Omim: 164730 Human
      • Omim: 164731 Human
      • Omim: 611223 Human
      • SwissProt: P31749 Human
      • SwissProt: P31751 Human
      • SwissProt: Q9Y243 Human
      • Unigene: 498292 Human
      • Unigene: 525622 Human
      • Unigene: 631535 Human
      see all
    • Alternative names

      • AKT antibody
      • AKT1 antibody
      • AKT1 kinase antibody
      • AKT1m antibody
      • AKT2 antibody
      • AKT2 kinase antibody
      • Akt3 antibody
      • AKT3_HUMAN antibody
      • CAKT antibody
      • CWS6 antibody
      • DKFZp434N0250 antibody
      • HIHGHH antibody
      • kinase Akt1 antibody
      • MGC99656 antibody
      • MPPH antibody
      • Murine thymoma viral (v-akt) homolog 2 antibody
      • pan-akt antibody
      • PKB ALPHA antibody
      • PKB antibody
      • PKB beta antibody
      • PKB gamma antibody
      • PKB-GAMMA antibody
      • PKB/Akt antibody
      • PKBALPHA antibody
      • PKBB antibody
      • PKBBETA antibody
      • PKBG antibody
      • PKBGAMMA antibody
      • PRKBA antibody
      • PRKBB antibody
      • PRKBG antibody
      • Protein kinase Akt 2 antibody
      • Protein kinase Akt-2 antibody
      • Protein kinase Akt-3 antibody
      • Protein kinase B alpha antibody
      • Protein kinase B antibody
      • Protein kinase B beta antibody
      • Protein kinase B gamma antibody
      • Proto oncogene c Akt antibody
      • Proto-oncogene c-Akt antibody
      • RAC ALPHA antibody
      • RAC alpha serine/threonine protein kinase antibody
      • RAC antibody
      • RAC BETA antibody
      • RAC beta serine/threonine protein kinase antibody
      • RAC PK alpha antibody
      • RAC PK beta antibody
      • rac protein kinase alpha antibody
      • rac protein kinase beta antibody
      • RAC-ALPHA antibody
      • RAC-alpha serine/threonine-protein kinase antibody
      • RAC-beta serine/threonine-protein kinase antibody
      • RAC-gamma antibody
      • RAC-gamma serine/threonine-protein kinase antibody
      • RAC-PK-alpha antibody
      • RAC-PK-beta antibody
      • RAC-PK-gamma antibody
      • RACALPHA antibody
      • RACalpha serine/threonine kinase antibody
      • RACBETA antibody
      • RACgamma antibody
      • RACgamma serine/threonine protein kinase antibody
      • RACPKgamma antibody
      • serine threonine protein kinase antibody
      • STK 2 antibody
      • STK-2 antibody
      • STK2 antibody
      • thymoma viral proto oncogene 1 antibody
      • thymoma viral proto oncogene antibody
      • V akt murine thymoma viral oncogene homolog 1 antibody
      • V akt murine thymoma viral oncogene homolog 2 antibody
      • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
      • V akt murine thymoma viral oncogene homolog 3 antibody
      • V-AKT murine thymoma viral oncogene homolog 1 antibody
      • V-AKT murine thymoma viral oncogene homolog 2 antibody
      • V-AKT murine thymoma viral oncogene homolog 3 antibody
      • vakt murine thymoma viral oncogene homolog 1 antibody
      • vakt murine thymoma viral oncogene homolog 2 antibody
      • vakt murine thymoma viral oncogene homolog 3 antibody
      see all

    Images

    • Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab184136)
      Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab184136)
      All lanes : Anti-AKT1 + AKT2 + AKT3 antibody (ab184136) at 1/1000 dilution

      Lane 1 : 293T whole cell lysate/extract
      Lane 2 : A431 whole cell lysate/extract
      Lane 3 : HeLa whole cell lysate/extract
      Lane 4 : HepG2 whole cell lysate/extract
      Lane 5 : A375 whole cell lysate/extract

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 56 kDa



      10 % SDS-PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab184136? Please let us know so that we can cite the reference in this datasheet.

    ab184136 has been referenced in 2 publications.

    • Yamaguchi M  et al. Prolactin Enhances the Proliferation of Proliferative Endometrial Glandular Cells and Endometrial Cancer Cells. J Endocr Soc 4:bvz029 (2020). PubMed: 32083234
    • Tayama S  et al. The impact of EpCAM expression on response to chemotherapy and clinical outcomes in patients with epithelial ovarian cancer. Oncotarget 8:44312-44325 (2017). PubMed: 28574829

    Customer reviews and Q&As

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