Anti-AKT1 + AKT2 + AKT3 antibody (ab184136)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-AKT1 + AKT2 + AKT3 antibody
See all AKT1 + AKT2 + AKT3 primary antibodies -
Description
Rabbit polyclonal to AKT1 + AKT2 + AKT3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human AKT1 + AKT2 + AKT3 aa 416-480. The exact sequence is proprietary.
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Positive control
- 293T, A431, HeLa, HepG2 and A375 whole cell lysate/extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 78% PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab184136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 56 kDa. |
Target
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Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
Post-translational
modificationsPhosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
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Database links
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Omim: 164730 Human
- Omim: 164731 Human
- Omim: 611223 Human
- SwissProt: P31749 Human
- SwissProt: P31751 Human
see all -
Alternative names
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
see all
Images
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Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab184136)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody (ab184136) at 1/1000 dilution
Lane 1 : 293T whole cell lysate/extract
Lane 2 : A431 whole cell lysate/extract
Lane 3 : HeLa whole cell lysate/extract
Lane 4 : HepG2 whole cell lysate/extract
Lane 5 : A375 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa10 % SDS-PAGE
Protocols
References
This product has been referenced in:
- Tayama S et al. The impact of EpCAM expression on response to chemotherapy and clinical outcomes in patients with epithelial ovarian cancer. Oncotarget 8:44312-44325 (2017). Read more (PubMed: 28574829) »
