Recombinant
RabMAb

Recombinant Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (Phycoerythrin) (ab210454)

Overview

  • Product name

    Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (Phycoerythrin)
    See all AKT1 + AKT2 + AKT3 primary antibodies
  • Description

    Rabbit monoclonal [EPR16798] to AKT1 + AKT2 + AKT3 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus tropicalis
  • Immunogen

    Recombinant fragment within Human AKT1 + AKT2 + AKT3 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P31749

  • Positive control

    • Flow Cyt: HeLa cells ICC/IF: HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab210454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2500.

ab209478 - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/500.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
  • Tissue specificity

    In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Domain

    Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
  • Post-translational
    modifications

    Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Cellular localization

    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • Database links

  • Alternative names

    • AKT antibody
    • AKT1 antibody
    • AKT1 kinase antibody
    • AKT1m antibody
    • AKT2 antibody
    • AKT2 kinase antibody
    • Akt3 antibody
    • AKT3_HUMAN antibody
    • CAKT antibody
    • CWS6 antibody
    • DKFZp434N0250 antibody
    • HIHGHH antibody
    • kinase Akt1 antibody
    • MGC99656 antibody
    • MPPH antibody
    • Murine thymoma viral (v-akt) homolog 2 antibody
    • pan-akt antibody
    • PKB ALPHA antibody
    • PKB antibody
    • PKB beta antibody
    • PKB gamma antibody
    • PKB-GAMMA antibody
    • PKB/Akt antibody
    • PKBALPHA antibody
    • PKBB antibody
    • PKBBETA antibody
    • PKBG antibody
    • PKBGAMMA antibody
    • PRKBA antibody
    • PRKBB antibody
    • PRKBG antibody
    • Protein kinase Akt 2 antibody
    • Protein kinase Akt-2 antibody
    • Protein kinase Akt-3 antibody
    • Protein kinase B alpha antibody
    • Protein kinase B antibody
    • Protein kinase B beta antibody
    • Protein kinase B gamma antibody
    • Proto oncogene c Akt antibody
    • Proto-oncogene c-Akt antibody
    • RAC ALPHA antibody
    • RAC alpha serine/threonine protein kinase antibody
    • RAC antibody
    • RAC BETA antibody
    • RAC beta serine/threonine protein kinase antibody
    • RAC PK alpha antibody
    • RAC PK beta antibody
    • rac protein kinase alpha antibody
    • rac protein kinase beta antibody
    • RAC-ALPHA antibody
    • RAC-alpha serine/threonine-protein kinase antibody
    • RAC-beta serine/threonine-protein kinase antibody
    • RAC-gamma antibody
    • RAC-gamma serine/threonine-protein kinase antibody
    • RAC-PK-alpha antibody
    • RAC-PK-beta antibody
    • RAC-PK-gamma antibody
    • RACALPHA antibody
    • RACalpha serine/threonine kinase antibody
    • RACBETA antibody
    • RACgamma antibody
    • RACgamma serine/threonine protein kinase antibody
    • RACPKgamma antibody
    • serine threonine protein kinase antibody
    • STK 2 antibody
    • STK-2 antibody
    • STK2 antibody
    • thymoma viral proto oncogene 1 antibody
    • thymoma viral proto oncogene antibody
    • V akt murine thymoma viral oncogene homolog 1 antibody
    • V akt murine thymoma viral oncogene homolog 2 antibody
    • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
    • V akt murine thymoma viral oncogene homolog 3 antibody
    • V-AKT murine thymoma viral oncogene homolog 1 antibody
    • V-AKT murine thymoma viral oncogene homolog 2 antibody
    • V-AKT murine thymoma viral oncogene homolog 3 antibody
    • vakt murine thymoma viral oncogene homolog 1 antibody
    • vakt murine thymoma viral oncogene homolog 2 antibody
    • vakt murine thymoma viral oncogene homolog 3 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab210454 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210454, 1/2500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

  • ab210454 staining AKT1 + AKT2 + AKT3 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210454 at 1/500 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

References

ab210454 has not yet been referenced specifically in any publications.

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