Recombinant Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Product name

Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671]
See all AKT1 + AKT2 + AKT3 primary antibodies
Description

Rabbit monoclonal [EPR17671] to AKT1 + AKT2 + AKT3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: AKT3 recombinant protein fragment (His-Tag®): aa351-479; AKT2 recombinant protein fragment (His-Tag®): aa282-481; AKT1 recombinant protein fragment (His-Tag®): aa281-480; A549 whole cell lysate; Human fetal brain and fetal kidney lysates; Mouse brain lysate; Rat brain and heart lysates. IHC-P: Human cerebral cortex, Human adenocarcinoma of colon, mouse cerebral cortex and rat kidney tissues. ICC/IF: HeLa cells. Flow Cytometry: A549 cells. IP: A549 whole cell extract.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17671
Isotype

IgG
Research areas

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Human

Application	Abreviews	Notes
Flow Cyt		1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP		1/50.
WB		1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P		1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
Flow Cyt 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/50.
WB 1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
Tissue specificity

In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Domain

Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
Post-translational
modifications

Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Cellular localization

Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Target information above from: UniProt accession Q9Y243 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 11652 Mouse
- Entrez Gene: 23797 Mouse
- Entrez Gene: 24185 Rat
- Entrez Gene: 25233 Rat
- Entrez Gene: 29414 Rat
- Omim: 164730 Human
- Omim: 164731 Human
- Omim: 611223 Human
- SwissProt: P31749 Human
- SwissProt: P31751 Human
- SwissProt: Q9Y243 Human
- SwissProt: P31750 Mouse
- SwissProt: Q60823 Mouse
- SwissProt: Q9WUA6 Mouse
- SwissProt: P47196 Rat
- SwissProt: P47197 Rat
- SwissProt: Q63484 Rat
- Unigene: 498292 Human
- Unigene: 525622 Human
- Unigene: 631535 Human
- Unigene: 177194 Mouse
- Unigene: 235194 Mouse
- Unigene: 6645 Mouse
- Unigene: 10506 Rat
- Unigene: 11422 Rat
- Unigene: 87066 Rat
see all
Alternative names
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
- AKT1m antibody
- AKT2 antibody
- AKT2 kinase antibody
- Akt3 antibody
- AKT3_HUMAN antibody
- CAKT antibody
- CWS6 antibody
- DKFZp434N0250 antibody
- HIHGHH antibody
- kinase Akt1 antibody
- MGC99656 antibody
- MPPH antibody
- Murine thymoma viral (v-akt) homolog 2 antibody
- pan-akt antibody
- PKB ALPHA antibody
- PKB antibody
- PKB beta antibody
- PKB gamma antibody
- PKB-GAMMA antibody
- PKB/Akt antibody
- PKBALPHA antibody
- PKBB antibody
- PKBBETA antibody
- PKBG antibody
- PKBGAMMA antibody
- PRKBA antibody
- PRKBB antibody
- PRKBG antibody
- Protein kinase Akt 2 antibody
- Protein kinase Akt-2 antibody
- Protein kinase Akt-3 antibody
- Protein kinase B alpha antibody
- Protein kinase B antibody
- Protein kinase B beta antibody
- Protein kinase B gamma antibody
- Proto oncogene c Akt antibody
- Proto-oncogene c-Akt antibody
- RAC ALPHA antibody
- RAC alpha serine/threonine protein kinase antibody
- RAC antibody
- RAC BETA antibody
- RAC beta serine/threonine protein kinase antibody
- RAC PK alpha antibody
- RAC PK beta antibody
- rac protein kinase alpha antibody
- rac protein kinase beta antibody
- RAC-ALPHA antibody
- RAC-alpha serine/threonine-protein kinase antibody
- RAC-beta serine/threonine-protein kinase antibody
- RAC-gamma antibody
- RAC-gamma serine/threonine-protein kinase antibody
- RAC-PK-alpha antibody
- RAC-PK-beta antibody
- RAC-PK-gamma antibody
- RACALPHA antibody
- RACalpha serine/threonine kinase antibody
- RACBETA antibody
- RACgamma antibody
- RACgamma serine/threonine protein kinase antibody
- RACPKgamma antibody
- serine threonine protein kinase antibody
- STK 2 antibody
- STK-2 antibody
- STK2 antibody
- thymoma viral proto oncogene 1 antibody
- thymoma viral proto oncogene antibody
- V akt murine thymoma viral oncogene homolog 1 antibody
- V akt murine thymoma viral oncogene homolog 2 antibody
- V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
- V akt murine thymoma viral oncogene homolog 3 antibody
- V-AKT murine thymoma viral oncogene homolog 1 antibody
- V-AKT murine thymoma viral oncogene homolog 2 antibody
- V-AKT murine thymoma viral oncogene homolog 3 antibody
- vakt murine thymoma viral oncogene homolog 1 antibody
- vakt murine thymoma viral oncogene homolog 2 antibody
- vakt murine thymoma viral oncogene homolog 3 antibody
see all

Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/5000 dilution

Lane 1 : AKT3 recombinant protein fragment (His-Tag®): aa351-479
Lane 2 : AKT2 recombinant protein fragment (His-Tag®): aa282-481
Lane 3 : AKT1 recombinant protein fragment (His-Tag®): aa281-480

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
The bottom panel is probed with an anti-His tag antibody.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/2000 dilution + A549 (Human lung carcinoma) whole cell lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/2000 dilution

Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal kidney lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/2000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lane 3 : Rat heart lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on neurons of the Human cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of colon tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on Human adenocarcinoma of colon is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on neurons of the mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on rat kidney is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

AKT1 + AKT2 + AKT3 was immunoprecipitated from 1mg of A549 (Human lung carcinoma) whole cell extract with ab185633 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab185633 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1: A549 whole cell extract 10 µg (Input). Lane 2: ab185633 IP in A549 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185633 in A549 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Flow Cytometry - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Flow cytometric analysis of 2% paraformaldehyde-fixed A549 (Human lung carcinoma) cells labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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Publishing research using ab185633? Please let us know so that we can cite the reference in this datasheet.

ab185633 has been referenced in 5 publications.

Zhang X et al. Emodin protects H9c2 cells from hypoxia-induced injury by up-regulating miR-138 expression. Braz J Med Biol Res 52:e7994 (2019). PubMed: 30810622
Wang M et al. Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3ß pathway. Int J Mol Med 43:2133-2143 (2019). PubMed: 30864694
Wang W et al. Differentiation antagonizing non-protein coding RNA modulates the proliferation, migration, and angiogenesis of glioma cells by targeting the miR-216a/LGR5 axis and the PI3K/AKT signaling pathway. Onco Targets Ther 12:2439-2449 (2019). PubMed: 31114219
Yi L et al. Down-regulation of HTR1A-modulated ACC activation contributes to stress-induced visceral hyperalgesia in rats. Neurogastroenterol Motil 31:e13620 (2019). PubMed: 31121088
Lu D et al. Ca2+/Mg2+ homeostasis-related TRPM7 channel mediates chondrocyte hypertrophy via regulation of the PI3K-Akt signaling pathway. Mol Med Rep 16:5699-5705 (2017). PubMed: 28849029

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (5)

Customer reviews and Q&As

Post-translational
modifications