Product nameAnti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474) antibody
See all AKT1 + AKT2 + AKT3 primary antibodies
DescriptionRabbit polyclonal to AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human AKT1 + AKT2 + AKT3 aa 416-480 (phospho S472 + S473 + S474). The exact sequence is proprietary.
- MCF7 whole cell lysate/extract (500nM PMA treatment).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 56 kDa.|
FunctionIGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
Tissue specificityIn adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
modificationsPhosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
- Information by UniProt
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
All lanes : Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474) antibody (ab183758) at 1/500 dilution
Lane 1 : MCF7 whole cell lysate/extract (untreated)
Lane 2 : MCF7 whole cell lysate/extract (500nM PMA treatment for 1hr)
Lane 3 : MCF7 whole cell lysate/extract (500nM PMA treatment for 2hrs)
Lane 4 : MCF7 whole cell lysate/extract (500nM PMA treatment for 4hrs)
Lane 5 : MCF7 whole cell lysate/extract (500nM PMA treatment for 6hrs)
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
7.5 % SDS-PAGE
This product has been referenced in:
- Wen Q et al. FoxO3a inhibiting expression of EPS8 to prevent progression of NSCLC: A new negative loop of EGFR signaling. EBioMedicine 40:198-209 (2019). Read more (PubMed: 30738830) »
- Wang X et al. Downregulation of GBAS regulates oral squamous cell carcinoma proliferation and apoptosis via the p53 signaling pathway. Onco Targets Ther 12:3729-3742 (2019). Read more (PubMed: 31190874) »