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RecombinantRabMAb

Recombinant Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

Submit a review Submit a question References (2)
Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
  • Immunoprecipitation - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
  • Dot Blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
  • ELISA - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
  • Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17680] to AKT1 (phospho S124)
  • Suitable for: ELISA, WB, IP, Dot blot
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-AKT1 (phospho S124) antibody [EPR17680]
    See all AKT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17680] to AKT1 (phospho S124)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, WB, IP, Dot blotmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, NIH/3T3, C6, RAW264.7 and PC12 whole cell lysates; Human fetal brain and fetal kidney lysates; mouse brain lysate; and rat brain and heart lysates. IP: MCF7 whole cell lysates. ELISA: AKT1 pS124 peptide.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/1700.
WB
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP
1/100.
Dot blot
Use at an assay dependent concentration.
Notes
ELISA
1/1700.
WB
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP
1/100.
Dot blot
Use at an assay dependent concentration.

Target

  • Function

    Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.

  • Tissue specificity

    Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.

  • Involvement in disease

    Defects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
    Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).

  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.

  • Domain

    Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
    The AGC-kinase C-terminal mediates interaction with THEM4.

  • Post-translational
    modifications

    Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.

  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AKT 1 antibody
    • AKT antibody
    • AKT1 antibody
    • AKT1_HUMAN antibody
    • C AKT antibody
    • cAKT antibody
    • MGC99656 antibody
    • PKB alpha antibody
    • PKB antibody
    • PKB-ALPHA antibody
    • PRKBA antibody
    • Protein Kinase B Alpha antibody
    • Protein kinase B antibody
    • Proto-oncogene c-Akt antibody
    • RAC Alpha antibody
    • RAC antibody
    • Rac protein kinase alpha antibody
    • RAC Serine/Threonine Protein Kinase antibody
    • RAC-alpha serine/threonine-protein kinase antibody
    • RAC-PK-alpha antibody
    • v akt murine thymoma viral oncogene homolog 1 antibody
    • vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody
    see all

Images

  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate, untreated
    Lane 2 : MCF7 whole cell lysate, treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 15 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/10000 dilution

    Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate, untreated
    Lane 2 : NIH/3T3 whole cell lysate, treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/10000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate, untreated
    Lane 2 : C6 whole cell lysate, treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunoprecipitation - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    Immunoprecipitation - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

    Immunoprecipitation of AKT1 from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate achieved using ab183556 at 1/100 dilution.
    Lane 1: Input: 10µg of MCF7 whole cell lysate.
    Lane 2: MCF7 whole cell lysate following IP with ab183556.
    Lane 3: negative control: IP using Rabbit monoclonal IgG (ab172730) instead of ab183556 in MCF7 whole cell lysate.
    Western blot was performed using ab183556 at 1/1000 dilution.
    An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 10 second exposure.

  • Dot Blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    Dot Blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

    Dot blot analysis of AKT1 (phospho S124) labeled with ab183556 at 1/1000 dilution.
    Lane 1: phospho peptide
    Lane 2: non phospho peptide
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.
    Exposure time: 3 minutes.

    Blocking and Diluting buffer buffer and concentration: 5% NFDM/TBST 

  • ELISA - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    ELISA - Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

    ELISA image showing specificity of ab183556 to AKT1 (phospho S124) peptide only.

    Peptides concentration: 1000 ng/ml.

    ab183556 working dilution: 1/1700.

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500.

  • Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)
    Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556)

References (2)

Publishing research using ab183556? Please let us know so that we can cite the reference in this datasheet.

ab183556 has been referenced in 2 publications.

  • Lim SH  et al. Depression-like behaviors induced by defective PTPRT activity through dysregulated synaptic functions and neurogenesis. J Cell Sci 133:N/A (2020). PubMed: 32938684
  • Zheng J  et al. Nrdp1 is involved in hippocampus apoptosis in cardiopulmonary bypass-induced cognitive dysfunction via the regulation of ErbB3 protein levels. Int J Mol Med 43:1747-1757 (2019). PubMed: 30720051

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