Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.7
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPeptide immunogen affinity column.
Our Abpromise guarantee covers the use of ab13991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionGeneral protein kinase capable of phosphorylating several known proteins.
Tissue specificityExpressed in all human cell types so far analyzed.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
modificationsPhosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
- Information by UniProt
- Akt2 antibody
- AKT2_HUMAN antibody
- HIHGHH antibody
Anti-AKT2 antibody (ab13991) at 1/500 dilution + mouse skin, whole tissue lysate at 4 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This product has been referenced in:
- Liu G et al. Kinase AKT1 negatively controls neutrophil recruitment and function in mice. J Immunol 191:2680-90 (2013). Read more (PubMed: 23904165) »
- Sully K et al. The mTOR inhibitor rapamycin opposes carcinogenic changes to epidermal Akt1/PKBa isoform signaling. Oncogene : (2012). Read more (PubMed: 22890326) »