Key features and details
- Rabbit polyclonal to AKT2
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-AKT2 antibody
See all AKT2 primary antibodies
DescriptionRabbit polyclonal to AKT2
Specificityab223617 does not react with AKT1 or AKT3.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to Human AKT2 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P31751
- WB: A43, A549, HCT 116, HeLa, HEK-293, HepG2, HL-60, HUVEC Jurkat, MCF7, PC-3 and T98G cell lysates; Rat brain lysate. ICC/IF: Heat shocked HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), Tris buffered saline
Concentration information loading...
PurityProtein A purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- Jurkat whole cell lysate (ab7899)
- HEK293 whole cell lysate (ab7902)
- A549 whole cell lysate (ab7910)
- HL60 whole cell lysate (ab7914)
Our Abpromise guarantee covers the use of ab223617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa).|
FunctionGeneral protein kinase capable of phosphorylating several known proteins.
Tissue specificityExpressed in all human cell types so far analyzed.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
modificationsPhosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
- Information by UniProt
- Akt2 antibody
- AKT2_HUMAN antibody
- HIHGHH antibody
2% formaledehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for AKT2 (green) using ab223617 at 1/25 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at room temperature.
(A) DAPI (blue) nuclear stain. (B) ab223617. (C) Merge.
Cells heat shocked at 42°C for 1 hour.
All lanes : Anti-AKT2 antibody (ab223617) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 7 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 8 : HUVEC (human umbilical vein endothelial cell line) cell lysate
Lane 9 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 10 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 11 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 12 : T98G cell lysate
Lane 13 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
All lanes : Donkey Anti-Rabbit IgG HRP
Predicted band size: 55 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Blocking: 1.5% BSA for 30 minutes at room temperature.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab223617 has not yet been referenced specifically in any publications.