Product nameAnti-AKT2 antibody
See all AKT2 primary antibodies
DescriptionRabbit polyclonal to AKT2
SpecificityThis product has been assayed by ELISA against 0.1 µg of the immunizing peptide. A 1/4000 to 1/16000 dilution of the antibody is recommended for this assay.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab9763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).|
FunctionGeneral protein kinase capable of phosphorylating several known proteins.
Tissue specificityExpressed in all human cell types so far analyzed.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
modificationsPhosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
- Information by UniProt
- Akt2 antibody
- AKT2_HUMAN antibody
- HIHGHH antibody
ab9763 used with a stable HEK293 cell line expressing an inducible, myristoylated form of Akt2 (MyrAkt2-ER). In response to 4OHT (tamoxifen), the Akt2 is recruited to the plasma membrane via its
myristoylation sequence and becomes phosphorylated and activated. The blot shows 0m, 15m, and 30m of 4OHT treatment (which has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2). Endogenous Akt2 runs at about 60kDa whereas the myristoylated construct runs at around 110kDa.
The second panel of 3 lanes is pretreated for 15min with 25uM LY294002 which affects the phosphorylation of endogenous Akt2 but has no effect on phosphorylation of the myristoylated construct. This confirms that the antibody picks up phosphorylated and non-phosphorylated forms of Akt2 whether it be endogenous or transfected in.
ab9763 used with a
ab9763 tested in Western blot with mouse tissues. This image was kindly provided as part of the review Jianping Ye. ab9763 tested in Western blot with mouse tissues. This image was kindly provided as part of the review Jianping Ye.
ab9763 has not yet been referenced specifically in any publications.