Overview

  • Product name

    Anti-AKT2 antibody [EP1676]
    See all AKT2 primary antibodies
  • Description

    Rabbit monoclonal [EP1676] to AKT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human AKT2 aa 1-100. The exact sequence is proprietary.
    Database link: P31751

  • Positive control

    • WB: Jurkat and 293T cell lysates.
  • General notes

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      General protein kinase capable of phosphorylating several known proteins.
    • Tissue specificity

      Expressed in all human cell types so far analyzed.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylation on Thr-309 and Ser-474 is required for full activity.
      Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
    • Information by UniProt
    • Database links

    • Alternative names

      • Akt2 antibody
      • AKT2_HUMAN antibody
      • HIHGHH antibody
      • Murine thymoma viral (v-akt) homolog 2 antibody
      • murine thymoma viral (v-akt) homolog-2 antibody
      • Oncogene AKT2 protein kinase B beta antibody
      • PKB antibody
      • PKB beta antibody
      • PKBB antibody
      • PKBBETA antibody
      • PRKBB antibody
      • Protein kinase Akt 2 antibody
      • Protein kinase Akt-2 antibody
      • Protein kinase B beta antibody
      • RAC beta antibody
      • rac protein kinase beta antibody
      • RAC-BETA antibody
      • RAC-beta serine/threonine-protein kinase antibody
      • RAC-PK-beta antibody
      • RACbeta antibody
      • v akt murine thymoma viral oncogene homolog 2 antibody
      • V-AKT murine thymoma viral oncogene homolog 2 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: AKT2 knockout HAP1 cell lysate (20 µg)
      Lanes 1 and 2: Merged signal (red and green). Green - ab131168 observed at 55 kDa. Red - loading control, ab8226, observed at 42 kDa.

      ab131168 was shown to recognize AKT2 when AKT2 knockout samples were used, along with additional cross-reactive bands. Wild-type and AKT2 knockout samples were subjected to SDS-PAGE. ab131168 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-AKT2 antibody [EP1676] (ab131168) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 55 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Duan C  et al. Sulfasalazine alters microglia phenotype by competing endogenous RNA effect of miR-136-5p and long non-coding RNA HOTAIR in cuprizone-induced demyelination. Biochem Pharmacol 155:110-123 (2018). Read more (PubMed: 29944870) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131168.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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