Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-AKT2 antibody [EP1676] - BSA and Azide free (ab226115)

Overview

  • Product name

    Anti-AKT2 antibody [EP1676] - BSA and Azide free
    See all AKT2 primary antibodies
  • Description

    Rabbit monoclonal [EP1676] to AKT2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human AKT2 aa 1-100. The exact sequence is proprietary.
    Database link: P31751

  • Positive control

    • WB: Jurkat and 293T cell lysates.
  • General notes

    Ab226115 is the carrier-free version of ab131168. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226115 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      General protein kinase capable of phosphorylating several known proteins.
    • Tissue specificity

      Expressed in all human cell types so far analyzed.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylation on Thr-309 and Ser-474 is required for full activity.
      Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
    • Information by UniProt
    • Database links

    • Alternative names

      • Akt2 antibody
      • AKT2_HUMAN antibody
      • HIHGHH antibody
      • Murine thymoma viral (v-akt) homolog 2 antibody
      • murine thymoma viral (v-akt) homolog-2 antibody
      • Oncogene AKT2 protein kinase B beta antibody
      • PKB antibody
      • PKB beta antibody
      • PKBB antibody
      • PKBBETA antibody
      • PRKBB antibody
      • Protein kinase Akt 2 antibody
      • Protein kinase Akt-2 antibody
      • Protein kinase B beta antibody
      • RAC beta antibody
      • rac protein kinase beta antibody
      • RAC-BETA antibody
      • RAC-beta serine/threonine-protein kinase antibody
      • RAC-PK-beta antibody
      • RACbeta antibody
      • v akt murine thymoma viral oncogene homolog 2 antibody
      • V-AKT murine thymoma viral oncogene homolog 2 antibody
      see all

    References

    ab226115 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab226115.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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