Product nameAnti-AKT3 + AKT1 antibody [E45]
See all AKT3+AKT1 primary antibodies
DescriptionRabbit monoclonal [E45] to AKT3 + AKT1
Specificityab32038 detects Akt1 and Akt3.
Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide corresponding to Human AKT1 aa 450-550.
Database link: P31749
- Squamous lung carcinoma
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. PubMed: 19679212|
|IHC-P||1/250 - 1/1000.|
Cellular localizationAKT3: Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. AKT1: Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma
All lanes : Anti-AKT3 + AKT1 antibody [E45] (ab32038) at 1/10000 dilution
Lane 1 : GFP tagged AKT1 transfected 293T cell lysate
Lanes 2 & 4 & 6 : Empty vector transfected 293T cell lysate
Lane 3 : GFP tagged AKT2 transfected 293T cell lysate
Lane 5 : GFP tagged AKT3 transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Exposure time: 1 second
Blocking/Diluting buffer: 5% NFDM/TBST.
Observed band: 82kDa, 56kDa.
82kDa band represents GFP tagged AKT. 56kDa band represents endogenous AKT.
This product has been referenced in:
- Li N et al. Cross-talk between TNF-a and IFN-? signaling in induction of B7-H1 expression in hepatocellular carcinoma cells. Cancer Immunol Immunother 67:271-283 (2018). Read more (PubMed: 29090321) »
- Yang CY et al. Melatonin exerts anti-oral cancer effect via suppressing LSD1 in patient-derived tumor xenograft models. Oncotarget 8:33756-33769 (2017). Read more (PubMed: 28422711) »