Anti-AKT3 antibody (ab152157)
Key features and details
- Rabbit polyclonal to AKT3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-AKT3 antibody
See all AKT3 primary antibodies -
Description
Rabbit polyclonal to AKT3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
-
Immunogen
Recombinant fragment corresponding to Human AKT3 aa 1-164.
Database link: Q9Y243 -
Positive control
- Recombinant Human AKT3 protein (ab43621) can be used as a positive control in WB. Human A549 xenograft tissue; 293T, A431, HepG2, MOLT4, Raji, HA-AKT transfected HeLa cells and mouse brain whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/500.
|
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
|
| Notes |
|---|
|
ICC/IF
1/500. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
Target
-
Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
Post-translational
modificationsPhosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
-
Database links
- Entrez Gene: 100137872 Cow
- Entrez Gene: 10000 Human
- Entrez Gene: 23797 Mouse
- Entrez Gene: 29414 Rat
- Omim: 611223 Human
- SwissProt: Q9Y243 Human
- SwissProt: Q9WUA6 Mouse
- SwissProt: Q63484 Rat
see all -
Alternative names
- Akt3 antibody
- AKT3 kinase antibody
- AKT3_HUMAN antibody
see all
Images
-
Western blot - Anti-AKT3 antibody (ab152157)All lanes : Anti-AKT3 antibody (ab152157) at 1/1000 dilution
Lane 1 : Non-transfected 293T whole cell extracts
Lane 2 : Transfected (+) 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 antibody (ab152157)Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue, staining AKT3 using ab152157 at a 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-AKT3 antibody (ab152157)Immunofluorescent analysis of methanol fixed MCF-7 cells labeling AKT3 with ab152157 at 1 in 500 dilution.
Blue: Hoechst 33342 staining.
-
Western blot - Anti-AKT3 antibody (ab152157)Anti-AKT3 antibody (ab152157) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE -
Western blot - Anti-AKT3 antibody (ab152157)Anti-AKT3 antibody (ab152157) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 54 kDa
7.5% SDS PAGE
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab152157 has been referenced in 9 publications.
- Zhao J et al. RHPN1-AS1 promotes cell proliferation and migration via miR-665/Akt3 in ovarian cancer. Cancer Gene Ther N/A:N/A (2020). PubMed: 32457485
- Cao L & Chai S miR-320-3p is involved in morphine pre-conditioning to protect rat cardiomyocytes from ischemia/reperfusion injury through targeting Akt3. Mol Med Rep 22:1480-1488 (2020). PubMed: 32468068
- Zhao C et al. MicroRNA-424-5p inhibits the proliferation, migration, and invasion of nasopharyngeal carcinoma cells by decreasing AKT3 expression. Braz J Med Biol Res 53:e9029 (2020). PubMed: 32520206
- Rossi F et al. Circ-ZNF609 regulates G1-S progression in rhabdomyosarcoma. Oncogene 38:3843-3854 (2019). PubMed: 30670781
- Chen Y et al. Testicular orphan receptor 4 promotes tumor progression and implies poor survival through AKT3 regulation in seminoma. Cancer Sci 109:384-394 (2018). PubMed: 29197138
- Li LY et al. Genetic Profiles Associated with Chemoresistance in Patient-Derived Xenograft Models of Ovarian Cancer. Cancer Res Treat N/A:N/A (2018). PubMed: 30428638
- Workman A et al. The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency. J Virol 92:N/A (2018). PubMed: 29321317
- Sarmento-Cabral A et al. Metformin Reduces Prostate Tumor Growth, in a Diet-Dependent Manner, by Modulating Multiple Signaling Pathways. Mol Cancer Res 15:862-874 (2017). PubMed: 28385910
- Teng Y et al. MicroRNA-29B (mir-29b) regulates the Warburg effect in ovarian cancer by targeting AKT2 and AKT3. Oncotarget 6:40799-814 (2015). WB, IHC . PubMed: 26512921