Anti-AKT3 antibody (ab152157)

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Overview

  • Product name

    Anti-AKT3 antibody
    See all AKT3 primary antibodies

  • Description

    Rabbit polyclonal to AKT3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow

  • Immunogen

    Recombinant fragment corresponding to Human AKT3 aa 1-164.
    Database link: Q9Y243

  • Positive control

    • Recombinant Human AKT3 protein (ab43621) can be used as a positive control in WB. Human A549 xenograft tissue; 293T, A431, HepG2, MOLT4, Raji, HA-AKT transfected HeLa cells and mouse brain whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab152157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

  • Function

    IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

  • Tissue specificity

    In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.

  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.

  • Domain

    Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.

  • Post-translational
    modifications

    Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.

  • Cellular localization

    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Akt3 antibody
    • AKT3 kinase antibody
    • AKT3_HUMAN antibody
    • DKFZp434N0250 antibody
    • MPPH antibody
    • PKB gamma antibody
    • PKBG antibody
    • PRKBG antibody
    • Protein kinase Akt-3 antibody
    • Protein Kinase AKT3 antibody
    • Protein kinase B gamma antibody
    • RAC gamma antibody
    • RAC gamma serine/threonine protein kinase antibody
    • RAC-gamma serine/threonine-protein kinase antibody
    • RAC-PK-gamma antibody
    • RACPK Gamma antibody
    • Serine threonine protein kinase Akt 3 antibody
    • Serine threonine protein kinase Akt3 antibody
    • STK 2 antibody
    • STK-2 antibody
    • STK2 antibody
    • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
    • V akt murine thymoma viral oncogene homolog 3 antibody
    • V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibody
    see all

Images

  • Western blot - Anti-AKT3 antibody (ab152157)
    Western blot - Anti-AKT3 antibody (ab152157)
    All lanes : Anti-AKT3 antibody (ab152157) at 1/1000 dilution

    Lane 1 : Non-transfected 293T whole cell extracts
    Lane 2 : Transfected (+) 293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 antibody (ab152157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 antibody (ab152157)
    Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue, staining AKT3 using ab152157 at a 1/500 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-AKT3 antibody (ab152157)
    Immunocytochemistry/ Immunofluorescence - Anti-AKT3 antibody (ab152157)

    Immunofluorescent analysis of methanol fixed MCF-7 cells labeling AKT3 with ab152157 at 1 in 500 dilution.

    Blue: Hoechst 33342 staining.

  • Western blot - Anti-AKT3 antibody (ab152157)
    Western blot - Anti-AKT3 antibody (ab152157)
    Anti-AKT3 antibody (ab152157) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 54 kDa



    10% SDS PAGE
  • Western blot - Anti-AKT3 antibody (ab152157)
    Western blot - Anti-AKT3 antibody (ab152157)
    Anti-AKT3 antibody (ab152157) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 54 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

  • Rossi F  et al. Circ-ZNF609 regulates G1-S progression in rhabdomyosarcoma. Oncogene 38:3843-3854 (2019). Read more (PubMed: 30670781) »
  • Chen Y  et al. Testicular orphan receptor 4 promotes tumor progression and implies poor survival through AKT3 regulation in seminoma. Cancer Sci 109:384-394 (2018). Read more (PubMed: 29197138) »
See all 6 Publications for this product

Publishing research using ab152157? Please let us know so that we can cite the reference in this datasheet.

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