Key features and details
- Rabbit polyclonal to AL5
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AL5 antibody
See all AL5 primary antibodies
DescriptionRabbit polyclonal to AL5
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human testis tissue. ICC/IF: A431 cells.
Previously labelled as PAGE1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
Tissue specificityIsolated from prostate cancer cell lines; expression associated with progression to androgen insensitive phenotype. Expressed in normal testis and at lower level in normal placenta.
Sequence similaritiesBelongs to the GAGE family.
- Information by UniProt
- AL5 antibody
- G antigen 9 antibody
- G antigen family B member 1 antibody
Paraffin-embedded human testis stained for AL5 using ab224329 at 1/200 dilution in immunohistochemical analysis. Shows moderate nuclear positivity in cells in seminiferous ducts.
Paraffin-embedded human gastrointestinal stained for AL5 using ab224329 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in glandular as expected.
Paraffin-embedded human cerebral cortex stained for AL5 using ab224329 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in neurons as expected.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for AL5 (green) using ab224329 at 4 µg/ml in ICC/IF.
ab224329 has not yet been referenced specifically in any publications.