Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10268(B)] to ALAD
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-ALAD antibody [EPR10268(B)]
See all ALAD primary antibodies
DescriptionRabbit monoclonal [EPR10268(B)] to ALAD
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human ALAD (P13716)
- Human red blood cells or fetal liver lysate, Human kidney tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab151754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 36 kDa.|
|IHC-P||1/50 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCatalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.
PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Involvement in diseaseDefects in ALAD are the cause of acute hepatic porphyria (AHP) [MIM:612740]. AHP is a form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AHP is characterized by attacks of gastrointestinal disturbances, abdominal colic, paralysis, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors.
Sequence similaritiesBelongs to the ALADH family.
- Information by UniProt
- ALAD antibody
- ALADH antibody
- ALADR antibody
All lanes : Anti-ALAD antibody [EPR10268(B)] (ab151754) at 1/1000 dilution
Lane 1 : Red blood cell lysate
Lane 2 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling ALAD with ab151754 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab151754 has been referenced in 2 publications.
- Maio N et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). PubMed: 30765471
- Vandekeere S et al. Serine Synthesis via PHGDH Is Essential for Heme Production in Endothelial Cells. Cell Metab N/A:N/A (2018). PubMed: 30017355