Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134)
- Datasheet
- Specific References (9)
- Protocols
Overview
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Product nameAlanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric)
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Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
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Assay typeQuantitative
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Sensitivity> 10 mU/well
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Product overview
Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134 is a rapid and simple assay where alanine transminase (ALT) catalyzes the transfer of an amino group from alanine to α-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (ODmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm).
The kit provides a rapid, simple, sensitive, and reliable test suitable for any samples containing ALT activity and has a detection limit of 10 mU per well.
Visit our FAQs page for tips and troubleshooting.
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Notes
Alanine transaminase is also called alanine aminotransferase or serum glutamic pyruvic transaminase (ALT, ALAT, SGPT).
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 100 tests ALT Positive Control (lyophilized) Blue 1 vial ALT Substrate (lyophilized) Orange 1 vial Pyruvate Standard (100 nmol/µl) Yellow 1 x 100µl ALT Assay Buffer WM 1 x 25ml ALT Enzyme Mix (lyophilized) Green 1 vial OxiRed™ (in DMSO Red 1 x 200µl -
Research areas
Images
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Determination of ALT activity using ab105134 Alanine Transaminase Activity Assay KitWaller-Evans H et al., PLoS One, 8(12). Fig 1d. doi: 10.1371/journal.pone.0082825Liver samples from high fat diet (HFD) and standard carbohydrate diet (CHD) BALB/c and C57BL6/J mice were homogenised in an ALT assay buffer for the determination of ALT activity using ab105134. A separate batch of liver extracts was prepared and incubated in a buffer containing NP40 (5%) and supernatants containing the triglycerides were separated. Triglycerides concentration was determined on the supernatant fraction using ab65336. ALT activity and triglycerides concentration were determined by measuring OD at 570nm.
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Functional Studies - Alanine Transaminase Activity Assay Kit (ab105134)Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted. -
Functional Studies - Alanine Transaminase Activity Assay Kit (ab105134)Fluorometric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
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Functional Studies - Alanine Transaminase Activity Assay Kit (ab105134)Alanine transaminase measured in mouse tissue lysates showing quantity (mU) per mg of tested sample.
Protein concentration for samples varied from 4 mg/mL to 13 mg/mL. Samples were diluted 9-27 fold and measured colorimetrically.
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Functional Studies - Alanine Transaminase Activity Assay Kit (ab105134)Pyruvate measured colorimetrically in cell lysate after 20 min and 40 min incubation time showing quantity (nmol) per 1 mln of tested cells.
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Functional Assay - ab105134Measurement of alanine transaminase in HepG2 cells (10 μg) and liver lysate (15 μg).
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Functional Studies - Alanine Transaminase Activity Assay Kit (105134)Pyruvate measured fluorometrically in cell lysate after 20 min and 40 min incubation time showing quantity (nmol) per 1 mln of tested cells.
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Functional Studies - Alanine Transaminase Activity Assay Kit (ab105134)Pyruvate measured in biological fluids after 20 min and 40 min incubation time showing quantity (nmol) per ml of tested sample.
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Functional Studies - Alanine Transaminase Activity Assay Kit (ab105134)Pyruvate measured in mouse tissue lysates after 20 min and 40 min incubation time showing quantity (nmol) per mg of tested sample.
Protocols
References
This product has been referenced in:
- Zia A et al. A case of a patient infected with a hepatitis C virus genotype 3a multidrug resistant variant in Pakistan. Infect Dis Poverty 7:11 (2018). Read more (PubMed: 29429413) »
- Hutt M et al. Targeting scFv-Fc-scTRAIL fusion proteins to tumor cells. Oncotarget 9:11322-11335 (2018). Read more (PubMed: 29541416) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"