Key features and details
- Rabbit polyclonal to Alanine Transaminase
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Alanine Transaminase antibody
See all Alanine Transaminase primary antibodies
DescriptionRabbit polyclonal to Alanine Transaminase
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Recombinant fragment, corresponding to a region within amino acids 6-188 of Human Alanine Transaminase (NP_005300).
- 293T, A431, H1299, HeLa, HepG2, Molt4, Raji, mouse liver whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 55 kDa.|
FunctionCatalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
Tissue specificityLiver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
PathwayAmino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
- Information by UniProt
- AAT1 antibody
- Alanine aminotransferase 1 antibody
- Alanine aminotransferase antibody
All lanes : Anti-Alanine Transaminase antibody (ab137531) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
10% SDS PAGE
Anti-Alanine Transaminase antibody (ab137531) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 55 kDa
7.5% SDS PAGE
ab137531 has not yet been referenced specifically in any publications.