Overview

  • Product name

    Anti-Alanine Transaminase antibody
    See all Alanine Transaminase primary antibodies
  • Description

    Goat polyclonal to Alanine Transaminase
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Alanine Transaminase aa 391-405 (internal sequence). NP_005300.1.
    Sequence:

    KLTEQVFNEAPGISC


    Database link: P24298

  • Positive control

    • WB: Human liver lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223506 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 54.6 kDa).

Target

  • Function

    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
  • Tissue specificity

    Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
  • Pathway

    Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
  • Sequence similarities

    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAT1 antibody
    • Alanine aminotransferase 1 antibody
    • Alanine aminotransferase antibody
    • ALAT1_HUMAN antibody
    • ALT1 antibody
    • Glutamate pyruvate transaminase 1 antibody
    • Glutamic alanine transaminase 1 antibody
    • Glutamic pyruvate transaminase (alanine aminotransferase) antibody
    • Glutamic pyruvic transaminase 1 antibody
    • Glutamic--alanine transaminase 1 antibody
    • Glutamic--pyruvic transaminase 1 antibody
    • GPT 1 antibody
    • gpt antibody
    • GPT1 antibody
    see all

Images

  • Anti-Alanine Transaminase antibody (ab223506) at 0.1 µg/ml + Human liver lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 54.6 kDa



    Primary incubation was 1 hour.

References

ab223506 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223506.
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