Anti-Alanine Transaminase antibody (ab231629)
Key features and details
- Rabbit polyclonal to Alanine Transaminase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Alanine Transaminase antibody
See all Alanine Transaminase primary antibodies -
Description
Rabbit polyclonal to Alanine Transaminase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment (His-tag) corresponding to Human Alanine Transaminase aa 350-500. (Expressed in E.coli).
Database link: P24298 -
Positive control
- IHC-P: Human liver tissue. WB: Human serum; Recombinant human Alanine Transaminase protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231629 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. -
Tissue specificity
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue. -
Pathway
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 539188 Cow
- Entrez Gene: 2875 Human
- Omim: 138200 Human
- SwissProt: A4IFH5 Cow
- SwissProt: P24298 Human
- Unigene: 103502 Human
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Alternative names
- AAT1 antibody
- Alanine aminotransferase 1 antibody
- Alanine aminotransferase antibody
see all
Images
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Paraffin-embedded human liver tissue stained for Alanine Transaminase using ab231629 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human liver tissue stained for Alanine Transaminase using ab231629 at 20 µg/ml in immunohistochemical analysis. AEC staining.
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Anti-Alanine Transaminase antibody (ab231629) at 3 µg/ml + Recombinant human Alanine Transaminase protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa -
Anti-Alanine Transaminase antibody (ab231629) at 3 µg/ml + Human serum
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231629 has not yet been referenced specifically in any publications.