Key features and details
- Sheep polyclonal to Alanine Transaminase
- Suitable for: Dot blot, WB, ELISA, IP
- Reacts with: Pig
- Isotype: IgG
Product nameAnti-Alanine Transaminase antibody
See all Alanine Transaminase primary antibodies
DescriptionSheep polyclonal to Alanine Transaminase
Tested applicationsSuitable for: Dot blot, WB, ELISA, IPmore details
Species reactivityReacts with: Pig
Full length protein from Pig heart.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by a delipidation and defibrination.
Our Abpromise guarantee covers the use of ab34832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1000 - 1/5000. This product has been assayed against 1.0 ug of Alanine Transaminase (ALT) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated affinity purified anti-Sheep IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at RT.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.
Suitable for most immunological methods requiring high titer and specificity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCatalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
Tissue specificityLiver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
PathwayAmino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
- Information by UniProt
- AAT1 antibody
- Alanine aminotransferase 1 antibody
- Alanine aminotransferase antibody
ab34832 has not yet been referenced specifically in any publications.