Recombinant
RabMAb

Recombinant Anti-Alanine Transaminase antibody [EPR19616] (ab202083)

Overview

  • Product name

    Anti-Alanine Transaminase antibody [EPR19616]
    See all Alanine Transaminase primary antibodies
  • Description

    Rabbit monoclonal [EPR19616] to Alanine Transaminase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Alanine Transaminase aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P24298

  • Positive control

    • WB: Human fetal liver and fetal kidney lysates; Human skeletal muscle, Mouse muscle, Rat muscle and Mouse liver lysates; HepG2 whole cell lysate; Mouse and rat brain and heart lysates. ICC/IF: HepG2 and HT-29 cells. Flow Cyt: HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab202083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF 1/100.
Flow Cyt 1/70.

Target

  • Function

    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
  • Tissue specificity

    Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
  • Pathway

    Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
  • Sequence similarities

    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAT1 antibody
    • Alanine aminotransferase 1 antibody
    • Alanine aminotransferase antibody
    • ALAT1_HUMAN antibody
    • ALT1 antibody
    • Glutamate pyruvate transaminase 1 antibody
    • Glutamic alanine transaminase 1 antibody
    • Glutamic pyruvate transaminase (alanine aminotransferase) antibody
    • Glutamic pyruvic transaminase 1 antibody
    • Glutamic--alanine transaminase 1 antibody
    • Glutamic--pyruvic transaminase 1 antibody
    • GPT 1 antibody
    • gpt antibody
    • GPT1 antibody
    see all

Images

  • All lanes : Anti-Alanine Transaminase antibody [EPR19616] (ab202083) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST..

  • All lanes : Anti-Alanine Transaminase antibody [EPR19616] (ab202083) at 1/2000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : Mouse muscle lysate
    Lane 4 : Rat muscle lysate
    Lane 5 : Mouse liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2, 3 and 4: 3 minutes; Lane 5: 4 seconds.

  • All lanes : Anti-Alanine Transaminase antibody [EPR19616] (ab202083) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 3 minutes; Lane 3 and 4: 30 seconds.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Alanine Transaminase with ab202083 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594 ) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202083 at 1/100 dilution followed by ab150120  at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling Alanine Transaminase with ab202083 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at  1/1000 dilution and Goat Anti-Mouse IgG  (AlexaFluor®594) preadsorbed (ab150120) at  1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202083 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Alanine Transaminase with ab202083 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody (blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

References

This product has been referenced in:

  • Li X  et al. Mitochondrial pyruvate carrier function determines cell stemness and metabolic reprogramming in cancer cells. Oncotarget 8:46363-46380 (2017). Read more (PubMed: 28624784) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Blocking
Sample
Mouse Recombinant protein (Recombinant Mouse ALT)
Specification
Recombinant Mouse ALT

Abcam user community

Verified customer

Submitted Jul 23 2019

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