Overview

  • Product name

  • Description

    Rabbit polyclonal to AlaRS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Hamster, Human
    Predicted to work with: Rat, Guinea pig, Cow
  • Immunogen

    Recombinant fragment corresponding to amino acids 695-968 (UniProt ID: Q8CFX8) of Hamster AlaRS with proprietary tag.

  • Positive control

    • HeLa and NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab139993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/100 - 1/1000. Predicted molecular weight: 107 kDa.

Target

  • Function

    Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
  • Involvement in disease

    Charcot-Marie-Tooth disease 2N
  • Sequence similarities

    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • Domain

    Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
    The C-terminal C-Ala domain (residues 756 to 968), along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. The human domain can be used in vitro to replace the corresponding domain in E.coli.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AARS antibody
    • AI316495 antibody
    • Alanine tRNA ligase 1, cytoplasmic antibody
    • Alanine tRNA ligase antibody
    • Alanine tRNA ligase cytoplasmic antibody
    • Alanine--tRNA ligase antibody
    • Alanyl tRNA synthetase antibody
    • Alanyl tRNA synthetase cytoplasmic antibody
    • Alanyl-tRNA synthetase antibody
    • AlaRS antibody
    • C76919 antibody
    • CMT2N antibody
    • cytoplasmic antibody
    • EC 6.1.1.7 antibody
    • MGC37368 antibody
    • Renal carcinoma antigen NY REN 42 antibody
    • Renal carcinoma antigen NY-REN-42 antibody
    • SYAC_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling AlaRS with ab139993 at 1/100 dilution. The cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% TritonX100. Alexa Fluor 488 conjugated goat anti-rabbit IgG antibody was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was stained with DAPI (left) and the merged image was shown in the center.

     

  • All lanes : Anti-AlaRS antibody (ab139993) at 1/100 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : NIH3T3 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 107 kDa

References

ab139993 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab139993.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up