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    alars-antibody-ab139993.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNA synthetase
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Anti-AlaRS antibody (ab139993)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-AlaRS antibody (ab139993)
  • Western blot - Anti-AlaRS antibody (ab139993)

Key features and details

  • Rabbit polyclonal to AlaRS
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-AlaRS antibody
    See all AlaRS primary antibodies
  • Description

    Rabbit polyclonal to AlaRS
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to amino acids 695-968 (UniProt ID: Q8CFX8) of Hamster AlaRS with proprietary tag.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa and NIH3T3 whole cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6
    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNA synthetase

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Rat
Guinea pig
Cow
Application Abreviews Notes
ICC/IF
1/100.
WB
1/100 - 1/1000. Predicted molecular weight: 107 kDa.
Notes
ICC/IF
1/100.
WB
1/100 - 1/1000. Predicted molecular weight: 107 kDa.

Target

  • Function

    Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
  • Involvement in disease

    Charcot-Marie-Tooth disease 2N
  • Sequence similarities

    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • Domain

    Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
    The C-terminal C-Ala domain (residues 756 to 968), along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. The human domain can be used in vitro to replace the corresponding domain in E.coli.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P49588 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 510933 Cow
    • Entrez Gene: 16 Human
    • Entrez Gene: 234734 Mouse
    • Entrez Gene: 292023 Rat
    • Omim: 601065 Human
    • SwissProt: P49588 Human
    • SwissProt: Q8BGQ7 Mouse
    • SwissProt: P50475 Rat
    • Unigene: 315137 Human
    • Unigene: 24174 Mouse
    • Unigene: 491269 Mouse
    • Unigene: 8645 Rat
    see all
  • Alternative names

    • AARS antibody
    • AI316495 antibody
    • Alanine tRNA ligase 1, cytoplasmic antibody
    • Alanine tRNA ligase antibody
    • Alanine tRNA ligase cytoplasmic antibody
    • Alanine--tRNA ligase antibody
    • Alanyl tRNA synthetase antibody
    • Alanyl tRNA synthetase cytoplasmic antibody
    • Alanyl-tRNA synthetase antibody
    • AlaRS antibody
    • C76919 antibody
    • CMT2N antibody
    • cytoplasmic antibody
    • EC 6.1.1.7 antibody
    • MGC37368 antibody
    • Renal carcinoma antigen NY REN 42 antibody
    • Renal carcinoma antigen NY-REN-42 antibody
    • SYAC_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-AlaRS antibody (ab139993)
    Immunocytochemistry/ Immunofluorescence - Anti-AlaRS antibody (ab139993)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling AlaRS with ab139993 at 1/100 dilution. The cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% TritonX100. Alexa Fluor 488 conjugated goat anti-rabbit IgG antibody was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was stained with DAPI (left) and the merged image was shown in the center.

     

  • Western blot - Anti-AlaRS antibody (ab139993)
    Western blot - Anti-AlaRS antibody (ab139993)
    All lanes : Anti-AlaRS antibody (ab139993) at 1/100 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : NIH3T3 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 107 kDa

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab139993? Please let us know so that we can cite the reference in this datasheet.

    ab139993 has not yet been referenced specifically in any publications.

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