Recombinant Anti-AlaRS antibody [EPR11036(B)] (ab166915)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-AlaRS antibody [EPR11036(B)]
See all AlaRS primary antibodies -
Description
Rabbit monoclonal [EPR11036(B)] to AlaRS -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to residues in Human AlaRS (UniProt P49588).
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Positive control
- HeLa, K562, Human fetal liver and HepG2 cell lysate. Permeabilized K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11036(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab166915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 107 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. -
Involvement in disease
Charcot-Marie-Tooth disease 2N -
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family. -
Domain
Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
The C-terminal C-Ala domain (residues 756 to 968), along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. The human domain can be used in vitro to replace the corresponding domain in E.coli. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 16 Human
- Entrez Gene: 234734 Mouse
- Entrez Gene: 292023 Rat
- Omim: 601065 Human
- SwissProt: P49588 Human
- SwissProt: Q8BGQ7 Mouse
- SwissProt: P50475 Rat
- Unigene: 315137 Human
see all -
Alternative names
- AARS antibody
- AI316495 antibody
- Alanine tRNA ligase 1, cytoplasmic antibody
see all
Images
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![Western blot - Anti-AlaRS antibody [EPR11036(B)] (ab166915)](https://www.abcam.com/ps/products/166/ab166915/Images/ab166915-165289-anti-alars-antibody-epr11036b-western-blot.jpg)
Western blot - Anti-AlaRS antibody [EPR11036(B)] (ab166915)All lanes : Anti-AlaRS antibody [EPR11036(B)] (ab166915) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : K562 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 107 kDa -
![Flow Cytometry - Anti-AlaRS antibody [EPR11036(B)] (ab166915)](https://www.abcam.com/ps/products/166/ab166915/Images/ab166915-165288-anti-alars-antibody-epr11036b-flow-cytometry.jpg)
Flow Cytometry - Anti-AlaRS antibody [EPR11036(B)] (ab166915)Flow cytometric analysis of permeabilized K562 cells labeling AlaRS with ab166915 at 1/10 (red) or a rabbit IgG control (negative) (green).
Datasheets and documents
References
ab166915 has not yet been referenced specifically in any publications.
