Overview

  • Product name
    Anti-AlaRS antibody [EPR11037(B)]
    See all AlaRS primary antibodies
  • Description
    Rabbit monoclonal [EPR11037(B)] to AlaRS
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human AlaRS (UniProt ID: P49588).

  • Positive control
    • Human fetal liver, HeLa, K562 and HepG2 lysates; endometrial adenocarcinoma tissue; kdney tissue; permeabilized K562 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR11037(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 107 kDa.
IHC-P 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
    • Involvement in disease
      Charcot-Marie-Tooth disease 2N
    • Sequence similarities
      Belongs to the class-II aminoacyl-tRNA synthetase family.
    • Domain
      Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
      The C-terminal C-Ala domain (residues 756 to 968), along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. The human domain can be used in vitro to replace the corresponding domain in E.coli.
    • Post-translational
      modifications
      ISGylated.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AARS antibody
      • AI316495 antibody
      • Alanine tRNA ligase 1, cytoplasmic antibody
      • Alanine tRNA ligase antibody
      • Alanine tRNA ligase cytoplasmic antibody
      • Alanine--tRNA ligase antibody
      • Alanyl tRNA synthetase antibody
      • Alanyl tRNA synthetase cytoplasmic antibody
      • Alanyl-tRNA synthetase antibody
      • AlaRS antibody
      • C76919 antibody
      • CMT2N antibody
      • cytoplasmic antibody
      • EC 6.1.1.7 antibody
      • MGC37368 antibody
      • Renal carcinoma antigen NY REN 42 antibody
      • Renal carcinoma antigen NY-REN-42 antibody
      • SYAC_HUMAN antibody
      see all

    Images

    • All lanes : Anti-AlaRS antibody [EPR11037(B)] (ab155275) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HeLa lysate
      Lane 3 : K562 lysate
      Lane 4 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 107 kDa

    • Immunohistochemical analysis of paraffin embedded Human endometrial adenocarcinoma tissue labeling AlaRS with ab155275 at a 1/50 dilution.
    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling AlaRS with ab155275 at a 1/50 dilution.
    • Flow cytometric analysis of permeabilized K562 cells using ab155275 at a 1/10 dilution (red) and a rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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