Recombinant
RabMAb

Recombinant Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)

Overview

  • Product name

    Anti-Alas1 antibody [EPR10247] - BSA and Azide free
    See all Alas1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10247] to Alas1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Alas1 aa 600 to the C-terminus. The exact sequence is proprietary.

  • General notes

    Ab211925 is the carrier-free version of ab154860. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab211925 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Pathway

      Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
    • Sequence similarities

      Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5 aminolevulinate synthase antibody
      • 5 aminolevulinate synthase nonspecific mitochondrial antibody
      • 5 aminolevulinic acid synthase antibody
      • 5-aminolevulinate synthase antibody
      • 5-aminolevulinic acid synthase 1 antibody
      • Alas 1 antibody
      • ALAS 3 antibody
      • ALAS antibody
      • ALAS H antibody
      • ALAS HOUSEKEEPING TYPE antibody
      • ALAS N antibody
      • ALAS-H antibody
      • alaS1 antibody
      • ALAS3 antibody
      • ALASH antibody
      • Aminolevulinate delta synthase 1 antibody
      • Aminolevulinic acid synthase 1 antibody
      • Delta ALA synthetase antibody
      • Delta aminolevulinate synthase antibody
      • Delta-ALA synthase 1 antibody
      • Delta-aminolevulinate synthase 1 antibody
      • HEM1_HUMAN antibody
      • MIG 4 antibody
      • MIG4 antibody
      • Migration inducing protein 4 antibody
      • mitochondrial antibody
      • nonspecific antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Alas1 with purified ab154860 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154860).

    • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling Alas1 with unpurified ab154860 at a dilution of 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154860).

    References

    ab211925 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab211925.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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