Recombinant
RabMAb

Recombinant Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)

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Overview

  • Product name

    Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker
    See all Alas1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10247] to Alas1 - Mitochondrial Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Alas1 aa 600 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • WB: HepG2, K562, JAR and Raji cell lysate. ICC/IF: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab154860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
ICC/IF 1/100 - 1/500.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Pathway

      Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.

    • Sequence similarities

      Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    • Database links

      • Alternative names

        • 5 aminolevulinate synthase antibody
        • 5 aminolevulinate synthase nonspecific mitochondrial antibody
        • 5 aminolevulinic acid synthase antibody
        • 5-aminolevulinate synthase antibody
        • 5-aminolevulinic acid synthase 1 antibody
        • Alas 1 antibody
        • ALAS 3 antibody
        • ALAS antibody
        • ALAS H antibody
        • ALAS HOUSEKEEPING TYPE antibody
        • ALAS N antibody
        • ALAS-H antibody
        • alaS1 antibody
        • ALAS3 antibody
        • ALASH antibody
        • Aminolevulinate delta synthase 1 antibody
        • Aminolevulinic acid synthase 1 antibody
        • Delta ALA synthetase antibody
        • Delta aminolevulinate synthase antibody
        • Delta-ALA synthase 1 antibody
        • Delta-aminolevulinate synthase 1 antibody
        • HEM1_HUMAN antibody
        • MIG 4 antibody
        • MIG4 antibody
        • Migration inducing protein 4 antibody
        • mitochondrial antibody
        • nonspecific antibody
        see all

      Images

      • Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)
        Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)
        Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/10000 dilution (purified) + HepG2 whole cell lysate at 10 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 71 kDa
        Observed band size: 71 kDa



        Blocking and dilution buffer: 5% NFDM/TBST
      • Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)
        Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)
        Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/10000 dilution (purified) + Raji whole cell lysate at 10 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 71 kDa
        Observed band size: 71 kDa



        Blocking and dilution buffer: 5% NFDM/TBST
      • Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)
        Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)

        Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Alas1 with purified ab154860 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

        Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

        Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

      • Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)
        Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)
        All lanes : Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/1000 dilution (unpurified)

        Lane 1 : HepG2 cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : JAR cell lysate
        Lane 4 : Raji cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 71 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)
        Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)

        Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling Alas1 with unpurified ab154860 at a dilution of 1/250.

      References

      This product has been referenced in:

      • Nakano T  et al. Hepatitis C virus core protein triggers abnormal porphyrin metabolism in human hepatocellular carcinoma cells. PLoS One 13:e0198345 (2018). Read more (PubMed: 29856826) »
      • Kubota Y  et al. Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. J Biol Chem N/A:N/A (2016). Read more (PubMed: 27496948) »
      See all 2 Publications for this product

      Publishing research using ab154860? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot abreview for Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HepG2 cells)
      Gel Running Conditions
      Reduced Denaturing (8% acrylamide gel)
      Loading amount
      10 µg
      Treatment
      with/without 0.1 mM succinylaceton
      Specification
      HepG2 cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 21 2016

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