Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Alas1 antibody [EPR10247] - Mitochondrial Marker
See all Alas1 primary antibodies -
DescriptionRabbit monoclonal [EPR10247] to Alas1 - Mitochondrial Marker
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Alas1 aa 600 to the C-terminus. The exact sequence is proprietary.
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Positive control
- WB: HepG2, K562, JAR and Raji cell lysate. ICC/IF: HepG2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR10247
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 71 kDa. | |
| ICC/IF | 1/100 - 1/500. |
Target
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PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
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Sequence similaritiesBelongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
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Cellular localizationMitochondrion matrix.
- Information by UniProt
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Database links
- Entrez Gene: 211 Human
- Omim: 125290 Human
- SwissProt: P13196 Human
- Unigene: 476308 Human
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Alternative names
- 5 aminolevulinate synthase antibody
- 5 aminolevulinate synthase nonspecific mitochondrial antibody
- 5 aminolevulinic acid synthase antibody
see all
Images
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![Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)](https://www.abcam.com/ps/products/154/ab154860/Images/ab154860-3-ab154860WB1.jpg)
Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/10000 dilution (purified) + HepG2 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)](https://www.abcam.com/ps/products/154/ab154860/Images/ab154860-4-ab154860WB2.jpg)
Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/10000 dilution (purified) + Raji whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
![Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)](https://www.abcam.com/ps/products/154/ab154860/Images/ab154860-5-ab154860ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Alas1 with purified ab154860 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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![Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)](https://www.abcam.com/ps/datasheet/images/154/ab154860/Alas1-Primary-antibodies-ab154860-1.jpg)
Western blot - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)All lanes : Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : JAR cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 71 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)](https://www.abcam.com/ps/datasheet/images/154/ab154860/Alas1-Primary-antibodies-ab154860-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860)Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling Alas1 with unpurified ab154860 at a dilution of 1/250.
References
This product has been referenced in:
- Kubota Y et al. Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. J Biol Chem N/A:N/A (2016). Read more (PubMed: 27496948) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"