Overview

  • Product name
  • Description
    Chicken polyclonal to Albumin
  • Host species
    Chicken
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Albumin (internal sequence).
    Database link: P02768

  • Positive control
    • Mouse liver lysate; Human liver tissue
  • General notes

    Antibodies should not be exposed to prolonged high temperatures.

Properties

Applications

Our Abpromise guarantee covers the use of ab106582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 69 kDa.
IHC-P Use a concentration of 2.5 - 20 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing.
  • Alternative names
    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • GIG20 antibody
    • GIG42 antibody
    • growth-inhibiting protein 20 antibody
    • OTTHUMP00000220436 antibody
    • OTTHUMP00000220438 antibody
    • OTTHUMP00000220439 antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • PRO1708 antibody
    • PRO2044 antibody
    • PRO2619 antibody
    • PRO2675 antibody
    • Serum albumin antibody
    • UNQ696/PRO1341 antibody
    see all

Images

  • Lane 1 : Anti-Albumin antibody (ab106582) at 1 µg/ml
    Lane 2 : Anti-Albumin antibody (ab106582) at 2 µg/ml

    All lanes : Mouse liver tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 69 kDa

  • Immunohistochemical analysis of paraffin embedded Human liver tissue section labelling Albumin with ab106582 at 2.5 µg/ml. 

  • Immunohistochemical analysis of paraffin embedded Human liver tissue section labelling Albumin with ab106582 at 20 µg/mL. 

  • Immunohistochemical analysis of paraffin embedded Mouse liver tissue section labelling Albumin with ab106582 at 5 µg/ml. 

References

This product has been referenced in:
  • Tagge CA  et al. Concussion, microvascular injury, and early tauopathy in young athletes after impact head injury and an impact concussion mouse model. Brain 141:422-458 (2018). Read more (PubMed: 29360998) »
  • Tong YF  et al. Maturity of associating liver partition and portal vein ligation for staged hepatectomy-derived liver regeneration in a rat model. World J Gastroenterol 24:1107-1119 (2018). Read more (PubMed: 29563755) »
See all 17 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (T98G Human brain glioblastoma)
Specification
T98G Human brain glioblastoma
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde

Dr. Dimitra Kalamida

Verified customer

Submitted Feb 03 2014

Answer

DISCOUNT CODE: *
*

Expiration date:*


I am very pleased to hear you would like to accept our offer and test ab106582/ab131176 in Flow Cytometry. This code will give you: 1 free PRIMARY ANTIBODY per discount codebefore the expiration date. To redeem this offer, please submit an Abreview forflow cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for contacting us.

Indeed, I am happy to let you know that we have anti-rat albumin antibodies (unconjugated and conjugated) available:
ab106582:Click here (or use the following: https://www.abcam.com/index.html?datasheet=106582).
ab53435: Click here (or use the following: https://www.abcam.com/index.html?datasheet=53435).
ab51422: Click here (or use the following: https://www.abcam.com/index.html?datasheet=51422).

Please be assured that all our products are covered by our Abpromise (www.abcam.com/Abpromise):

We guarantee our products work in the tested species and applications stated on the datasheet.
We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase.
100% Scientific and Customer Support (also in Spanish language!) for any product you buy from Abcam or one of our authorized distributors.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thanks for your reply. Either one of these 2 anti-albumin antibodies may work in flow although neither has yet been tested in that application. However we can't guarantee that they will work in flow. As we can't offer our Abpromise guarantee for flow with ab106582 or ab106784, you may be interested in the testing discount program described below. For purposes of the example I have used ab106582 but you can use this program with either antibody (or both): To our knowledge, ab/xxx/ has not been tested in flow cytometry. I can offer a discount off a future purchase if you buy ab106582 now, test it in flow cytometry and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab106582 in flow cytometry. I will then send a discount code. This code must be issued before purchasing ab106582 so please wait for my reply before ordering. 2. Purchase ab106582 either by phone, fax, or online (www.abcam.com). 3. Test it in flow cytometry. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab106582 turns out to be unsuitable for flow cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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