Overview

  • Product name

  • Description

    Rabbit polyclonal to Albumin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Albumin aa 540-569 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P02768

  • Positive control

    • WB: Mouse liver tissue lysate Flow Cyt: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab135575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 69 kDa.

Albumin is not endogenously expressed in 293 cells hence these shouldn't be used as positive control.

Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • GIG20 antibody
    • GIG42 antibody
    • growth-inhibiting protein 20 antibody
    • OTTHUMP00000220436 antibody
    • OTTHUMP00000220438 antibody
    • OTTHUMP00000220439 antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • PRO1708 antibody
    • PRO2044 antibody
    • PRO2619 antibody
    • PRO2675 antibody
    • Serum albumin antibody
    • UNQ696/PRO1341 antibody
    see all

Images

  • Anti-Albumin antibody (ab135575) at 1/1000 dilution + Mouse liver tissue lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 69 kDa



    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Albumin antibody (ab135575) at 1/1000 dilution + 293 cell lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 69 kDa



    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow cytometric analysis of HepG2 cells labelling Alubmin with ab135575 (green) compared to a negative control cell (blue). Cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the antibody (ab135575, 1µg/1x10^6 cells) for 60 min at 37ºC. The secondary antibody used was a FITC-conjugated goat anti-rabbit IgG at 1/200 dilution for 40 min at room temperature. Blank control (blue line) was used under the same conditions.Acquisition of >10,000 events was performed.

References

This product has been referenced in:

  • Lipps C  et al. Expansion of functional personalized cells with specific transgene combinations. Nat Commun 9:994 (2018). Read more (PubMed: 29520052) »
  • Xiang Y  et al. Effect of Yi Guan Jian decoction on differentiation of bone marrow mesenchymalstem cells into hepatocyte-like cells in dimethylnitrosamine-induced liver cirrhosis in mice. Mol Med Rep 15:613-626 (2017). Read more (PubMed: 28035356) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Mouse Serum (mouse blood serum)
Gel Running Conditions
Reduced Denaturing
Loading amount
0 µg
Specification
mouse blood serum
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 29 2016

Answer

Thank you for your enquiry.



Here are the answers to your quesitons.



1) "Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 547-577 of Human Albumin." means that the immunogen (amino acid range) used was taken from 547-577 of Human Albumin. So the antibody is specific to part of the human albumin chain.



2) I am not sure what you mean by passivization. Do you mean blocking solution? If so, you can also use the serum from the secondary antibody host species. In this case, if you are going to use ab96883, then please use 10% goat serum for blocking.



3) You did not mention what application you will be using the products in, so if it is a fluorescence related application like ICC/IF or Flow cytometry then yes, you can use this "Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488) (ab96883)" with ab135575.



If there is anything else that I can help you with please do not hesitate to contact me.


Have a nice day!

Read More

Answer

Thank you for contacting us.

In this case a casein block was used as BSA and serums would cause high background. We do carry a casein based blocking solution, ab126587,which we have used extensively and feel is a superior product in ICC. More information about ab126587 may be found at the following link:

https://www.abcam.com/10x-blocking-buffer-ab126587.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting Abcam.

I've compiled a list of primary antibodies that have been tested in mouse tissue via western blot. The only ovary-specific antibodies that I found were anti-human (with only 50% homology with the respective mouse protein), so the ovary antibody listed below is not exclusively-specific to ovary but also gives a positive signal in kidney, placenta, and small intestine:

1. Liver: anti-albumin ab135575 (https://www.abcam.com/albumin-antibody-c-terminal-ab135575.html)
2. Brain: anti-neuron specific beta III tubulin ab18207 (https://www.abcam.com/neuron-specific-beta-iii-tubulin-antibody-neuronal-marker-ab18207.html)
3. Kidney: anti-SLC14A2 ab95365 (https://www.abcam.com/slc14a2-antibody-ab95365.html)
4. Testis: anti-histone H2B ab23913 (https://www.abcam.com/histone-h2b-testis-specific-antibody-ab23913.html)
5. Ovary: anti-ENPP2 (also kidney, placenta, small intestine): ab77104 (https://www.abcam.com/enpp2-antibody-5h3-ab77104.html)

The advantage of this combination of antibodies is that you can use the same anti-rabbit secondary since they were all raised in a rabbit host. Our anti-rabbit secondary antibodies are listed in the link below:
https://www.abcam.com/Search?Keywords=anti-rabbit&fReset=1&pt=-1&source=TopSearch

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link:
https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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