Recombinant
RabMAb

Recombinant Anti-Albumin antibody [EPR20195] (ab207327)

Overview

  • Product name

    Anti-Albumin antibody [EPR20195]
    See all Albumin primary antibodies
  • Description

    Rabbit monoclonal [EPR20195] to Albumin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Albumin aa 1 to the C-terminus. (Purified Proteins from Normal Serum).
    Database link: P02768

  • Positive control

    • WB: Human, mouse and rat liver and plasma lysates. Human serum lysates. Human fetal kidney and spleen lysates. Mouse and rat spleen and kidney lysates. HepG2, NIH/3T3 and PC-12 whole cell lysates. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells. IP: HepG2 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab207327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ICC/IF 1/500.
IP 1/40.
Flow Cyt 1/500.

Permeabilization is required.

Target

  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • GIG20 antibody
    • GIG42 antibody
    • Growth inhibiting protein 20 antibody
    • growth-inhibiting protein 20 antibody
    • OTTHUMP00000220436 antibody
    • OTTHUMP00000220438 antibody
    • OTTHUMP00000220439 antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • PRO1708 antibody
    • PRO2044 antibody
    • PRO2619 antibody
    • PRO2675 antibody
    • Serum albumin antibody
    • UNQ696/PRO1341 antibody
    see all

Images

  • All lanes : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution

    Lane 1 : Human liver lysate at 20 µg
    Lane 2 : Mouse liver lysate at 20 µg
    Lane 3 : Rat liver lysate at 20 µg
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
    Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-3: 5 seconds; Lane 4/5: 1 second.

    Negative control: HeLa (PMID: 10476216 and 8314088).

  • Lanes 1-4 : Anti-Albumin antibody [EPR20195] (ab207327) at 1/10000 dilution
    Lanes 5-6 : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution

    Lane 1 : Human plasma at 2 µg
    Lane 2 : Rat plasma at 2 µg
    Lane 3 : Mouse plasma at 2 µg
    Lane 4 : Human serum at 2 µg
    Lane 5 : Human fetal kidney lysate at 10 µg
    Lane 6 : Human fetal spleen lysate at 10 µg

    Secondary
    Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 5-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : Rat kidney lysate
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 1 second; Lane 5: 3 minutes; Lane 6: 5 seconds.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) or HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Albumin with ab207327 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on HepG2 cell line.

    Negative control: HeLa (PMID: 10476216 and 8314088)).

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Albumin with ab207327 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Albumin was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207327 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab207327 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate, 10µg (Input).

    Lane 2: ab207327 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207327 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Albumin antibody ab207327 was used with 3D Cell Culture Clearing Kit ab243299 to penetrate, stain and clear a 3D hepatocyte cell culture.

    Blue: DAPI, Green: CD68, Yellow: Albumin, Red: Vimentin

    Learn more about 3D cell culture and tissue clearing kits, reagents, and protocols designed to make it easier to stain 3D cell cultures and thick tissue sections and get more data from each valuable tissue section.

References

This product has been referenced in:

  • Li YS  et al. Generation of H11-albumin-rtTA Transgenic Mice: A Tool for Inducible Gene Expression in the Liver. G3 (Bethesda) 9:591-599 (2019). Read more (PubMed: 30591434) »
  • Chen X  et al. Decoy receptor-3 regulates inflammation and apoptosis via PI3K/AKT signaling pathway in coronary heart disease. Exp Ther Med 17:2614-2622 (2019). Read more (PubMed: 30906453) »
See all 10 Publications for this product

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Mouse Tissue (brain)
Specification
brain

Abcam user community

Verified customer

Submitted Aug 28 2019

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