Product nameAnti-Albumin antibody (HRP)
See all Albumin primary antibodies
DescriptionSheep polyclonal to Albumin (HRP)
Tested applicationsSuitable for: IHC-P, WB, ELISA, ICCmore details
Species reactivityReacts with: Horse
Immunogen is purified horse albumin of serum or plasma origin
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.1% CMIT/MIT 3:1
Constituents: 0.2% BSA, 99.7% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was solid phase adsorbed to ensure class specificity. ab112976 was isolated by affinity chromatography using antigen coupled to agarose beads.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab112976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500.|
|WB||1/1000 - 1/30000. Predicted molecular weight: 69 kDa. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution.|
|ELISA||1/10000 - 1/100000.|
|ICC||1/200 - 1/500.|
FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
ab112976 has not yet been referenced specifically in any publications.