Overview

  • Product name

    Albumin (BCG) Assay Kit (Colorimetric)
    See all Albumin kits
  • Detection method

    Colorimetric
  • Sample type

    Serum
  • Assay type

    Quantitative
  • Sensitivity

    5 µg
  • Species reactivity

    Reacts with: Mammals
  • Product overview

    Albumin (BCG) Assay Kit (Colorimetric) (ab235628) is a simple high-throughput assay that detects Albumin concentration in serum.


    The assay is based on the selective interaction between Bromocresol Green (BCG) and albumin forming a chromophore that can be detected at 620 nm. The signal is directly proportional to the amount of albumin present in the serum. BCG does not react with other abundant plasma proteins like IgG.


    The assay can detect as low as 5 µg (0.01 g/dL) of albumin in serum samples.

  • Notes

    Albumin is the most abundant protein in human blood and is highly conserved among vertebrates. It plays a pivotal physiological role in maintenance of plasma osmotic pressure, vascular permeability, and transport of cholesterol, bile pigments, nitric oxide, metals, and other small molecules in the body. It also functions as a free radical scavenger of reactive oxygen and nitrogen species, triggers cell signaling processes, possesses anti-inframmatory and coagulatory effects.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Albumin Assay Buffer 1 x 25ml
    Bovine Serum Albumin (BSA, 50 mg/ml)  1 x 0.5ml
    Bromocresol Green (BCG) 1 x 100µl
  • Research areas

  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • alb
    • ALBU_HUMAN
    • Albumin (32 AA)
    • Albumin (AA 34)
    • cell growth inhibiting protein 42
    • DKFZp779N1935
    • GIG20
    • GIG42
    • Growth inhibiting protein 20
    • growth-inhibiting protein 20
    • OTTHUMP00000220436
    • OTTHUMP00000220438
    • OTTHUMP00000220439
    • PRO0883
    • PRO0903
    • PRO1341
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • Serum albumin
    • UNQ696/PRO1341
    see all

Images

  • Sample volumes (0-20 µL) were assayed following the kit protocol. Albumin concentration (g/dL): PHS: 3.8 ± 0.4; FBS: 1.6 ± 0.1; BCS: 2.6 ± 0.5.

  • BSA Standard Curve (0-75 µg).

  • BSA Standard Curve (0-500 µg).

Protocols

References

ab235628 has not yet been referenced specifically in any publications.

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