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Cardiovascular Blood Serum Proteins
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Albumin Creatinine Ratio Assay Kit (ab241018)

  • Datasheet
  • SDS
  • Protocol Booklet
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BSA Standard Curve.
  • Creatinine Standard Curve.
  • Estimation of ACR in human urine in diabetic (1) and non-diabetic donors (2, and 3).

Key features and details

  • Detection method: Fluorescent

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Overview

  • Product name

    Albumin Creatinine Ratio Assay Kit
  • Detection method

    Fluorescent
  • Product overview

    The Albumin Creatinine Ratio Assay Kit (ab241018) provides a simple, sensitive, and high throughput adaptable assay that detects albumin (detection range: 0.02- 2.5 mg/mL), creatinine (detection range: 0.002 -0.5 mg/mL) and Albumin-to-creatinine ratio.


    The ACR ratio is determined in two steps: First, albumin is determined by using a probe (AB580) that specifically recognizes albumin (Ex/Em = 600/630 nm). Second, creatinine is converted to sarcosine via enzymatic reactions.  Arcosine is specifically oxidized generating a product that reacts with a probe producing a chromophore that can be detected at 570 nm.


     

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Albumin Assay Buffer 1 x 7ml
    Albumin Diluent 1 x 7ml
    Albumin Probe (AB580) 1 x 0.4ml
    BSA Standard (2 mg/ml) 1 x 1ml
    Creatinase 1 vial
    Creatine Standard (10 µmol) (Lyophilized) Yellow 1 vial
    Creatininase 1 vial
    Creatinine Assay Buffer 1 x 25ml
    Creatinine Enzyme Mix 1 vial
    Creatinine Probe 1 x 0.2ml
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    • Metabolism
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  • Cellular localization

    Albumin: Secreted.

Associated products

    Images

    • BSA Standard Curve.
      BSA Standard Curve.
      BSA Standard Curve.
    • Creatinine Standard Curve.
      Creatinine Standard Curve.
      Creatinine Standard Curve.
    • Estimation of ACR in human urine in diabetic (1) and non-diabetic donors (2, and 3).
      Estimation of ACR in human urine in diabetic (1) and non-diabetic donors (2, and 3).

      For Albumin, 50 µL of undiluted samples and for Creatinine, 30 µL of diluted samples (100 times diluted using Creatinine Assay Buffer) were assayed following the kit protocol.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab241018? Please let us know so that we can cite the reference in this datasheet.

    ab241018 has not yet been referenced specifically in any publications.

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