Overview

  • Product name

    Albumin Rabbit ELISA Kit
    See all Albumin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.8%
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    > 0.19 µg/ml
  • Range

    0.4 µg/ml - 40 µg/ml
  • Recovery

    98 %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rabbit
  • Product overview

    Abcam’s Albumin rabbit in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rabbit Albumin in plasma, serum, urine, and cell culture supernatant.


    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with wash buffer. Strepatvidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Strepatvidin-Peroxidase enzymatic reaction. TMB is catalyzed by Strepatvidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • Representative standard curve using ab108793

Protocols

References

ab108793 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

The pre-coated capture antibody is a chicken polyclonal antibody.

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Answer

Thank you for your inquiry.


This kit does come with a control of sorts. We include one unit of rabbit albumin which is used for the standard curve.


https://www.abcam.com/Albumin-Rabbit-ELISA-Kit-ab108793.html


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I hope this information helps. Please contact us with any other questions.

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