Overview

  • Product name

    Alcian Blue Stain Kit (pH 1.0, Mucin Stain)
    See all strongly sulfated mucins kits
  • Product overview

    The Alcian Blue (pH 1.0) (Mucin Stain)  is intended for use in the histological visualization of strongly sulfated mucosubstances.


    Alcian Blue staining protocol summary:
    - deparaffinize sections if necessary and hydrate
    - incubate in acetic acid for 3 min
    - incubate in Alcian Blue pH 1.0 for 30 min
    - rinse in water
    - stain in salfranin O for 5 min
    - rinse in water
    - dehydrate, clear and mount 


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Notes

    Staining Interpretation 

    Strongly Sulfated Mucosubstances      Blue
    Nuclei Red
    Background Pink

    Control Tissue: Small Intestine, Appendix, Colon

    Previously called Alcian Blue pH 1.0 Stain Kit (Mucin Stain)

Properties

Images

  • ab150661 Alcian Blue pH 1 Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human GI tract.

  • ab150661 (Alcian Blue pH 1.0) staining strongly sulphated mucins (blue) in formalin fixed paraffin embedded human colon, counterstained with safranin o (pink).

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Protocols

References

ab150661 has not yet been referenced specifically in any publications.

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