Overview

  • Product name

  • Product overview

    Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).


    This Kit originally used Safranin O Solution but this has now been replaced with Nuclear Fast Red Solution.


    Alcian Blue staining protocol summary:
    - deparaffinize sections if necessary and hydrate
    - incubate in acetic acid for 3 min
    - incubate in Alcian Blue pH 2.5 for 30 min
    - rinse in water
    - stain in nuclear fast red for 5 min
    - rinse in water
    - dehydrate, clear and mount 


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Notes

    Staining Interpretation 

    Acidic Sulfated Mucosubstances    Blue
    Hyaluronic Acid Blue
    Sialomucins Blue
    Nuclei Red
    Background Pink

    Control Tissue: Small Intestine, Appendix, Colon

     

    Previously called Alcian Blue pH 2.5 Stain Kit (Mucin Stain)

Properties

Associated products

Images

  • ab150662 Alcian Blue pH 2.5 Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human GI tract.

  • ab150662 (Alcian Blue pH2.5 stain) staining acidic sulphated mucins (blue) in formalin fixed paraffin embedded normal human colon, counterstained with safranin o (pink).

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Staining of Colon using ab150662 - Alcian Blue pH 2.5 Stain Kit (Mucin Stain).

Protocols

References

This product has been referenced in:

  • Kuo YR  et al. Recipient Adipose-Derived Stem Cells Enhance Recipient Cell Engraftment and Prolong Allotransplant Survival in a Miniature Swine Hind-Limb Model. Cell Transplant 26:1418-1427 (2017). Read more (PubMed: 28901186) »
See 1 Publication for this product

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