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Since the results were so uncertain, I tried only once again with a new cell line that I am sure to have both the enzymes (ADH and CYP2E1) and actually I obtained a strong band. Unfortunately not only one but many and with similar MW. Probably I have to go further down with the concentration of the primary antibody but at the moment I have to do other experiments, so I will do it in the future months. Moreover, I don't have fresh tissue avaiable and I used it only as a control so now that I found an alternative positive control (the cells aforementioned) I can't spend other time on the tissue.I would like anyway to thank you both for your help. Moreover, I am beginning with immunofluorescence and I have seen that you have a new book about IHC/IF protocols for your customers. In the last 6 months we have bought at least 4 antibodies and I will need more in the future, but not before October, so I would like to ask you if it is possible to have the book anyway... Thank you very much again. Best regards
Asked on Sep 21 2011
Thank you for your email. I am very sorry for your disappointment. I do appreciate your cooperation in this whole case which actually happened to be a strange as well as interesting. I am presenting this case to my whole department next week to know what other scientists are thinking about the problem. I will forward you the summary of the discussion. In future if you need any assistance from us we will be very happy to help. Regarding the IHC book I am sorry only the new secondary antibodies orders are eligible for this book. This is a good book so I would suggest buying the antibody now and using it within 6 month time period which is the Abcam product guarantee period. In case antibody fails you can always call us for free of charge replacement. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Sep 21 2011