Recombinant Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
![Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)](https://www.abcam.com/ps/products/108/ab108203/Images/ab108203-120705-anti-alcohol-dehydrogenase-antibody-epr4439-western-blot.jpg "Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Alcohol Dehydrogenase antibody [EPR4439]
See all Alcohol Dehydrogenase primary antibodies -
Description
Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: lysate from HepG2 cells, Human fetal, mouse or rat liver; IHC-P: Human liver tissue; ICC/IF: HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4439 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (3) |
1/500 - 1/1000.
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| WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
|
| Notes |
|---|
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ICC/IF
1/500 - 1/1000. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Target
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Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 124 Human
- Entrez Gene: 11522 Mouse
- Entrez Gene: 24172 Rat
- Omim: 103700 Human
- SwissProt: P07327 Human
- SwissProt: P00329 Mouse
- SwissProt: P06757 Rat
- Unigene: 654433 Human
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Alternative names
- ADH alpha subunit antibody
- ADH antibody
- ADH1 antibody
see all
Images
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Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Rat liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)](https://www.abcam.com/ps/products/108/ab108203/Images/ab108203-236639-anti-alcohol-dehydrogenase-antibody-epr4439-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)This image is courtesy of an Abreview submitted by Armen Petrosyanab108203 staining Alcohol Dehydrogenase in the HepG2 cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50) for 1 hour at 22°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody. Nucleus was stained with DAPI (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)](https://www.abcam.com/ps/products/108/ab108203/Images/ab108203-120704-anti-alcohol-dehydrogenase-antibody-epr4439-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)ab108203 at 1/250 dilution staining Alcohol Dehydrogenase in paraffin-embedded Human liver tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)](https://www.abcam.com/ps/products/108/ab108203/Images/ab108203-120703-anti-alcohol-dehydrogenase-antibody-epr4439-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)ab108203 at 1/500 dilution staining Alcohol Dehydrogenase in HepG2 cells by Immunofluorescence. -
![Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)](https://www.abcam.com/ps/products/108/ab108203/Images/ab108203-5-benefits-of-recombinant-antibodies.png)
Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab108203 has been referenced in 5 publications.
- Manca S et al. The Role of Alcohol-Induced Golgi Fragmentation for Androgen Receptor Signaling in Prostate Cancer. Mol Cancer Res 17:225-237 (2019). PubMed: 30224543
- Sonoda H et al. The retinol-retinoic acid metabolic pathway is impaired in the lumbar spine of a rat model of congenital kyphoscoliosis. J Cell Biochem 120:15007-15017 (2019). PubMed: 31056772
- Gao Q et al. Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma. Cell 179:561-577.e22 (2019). PubMed: 31585088
- De Logu F et al. Schwann cells expressing nociceptive channel TRPA1 orchestrate ethanol-evoked neuropathic pain in mice. J Clin Invest 129:5424-5441 (2019). PubMed: 31487269
- Sengupta M et al. Inhibition of Hepatotoxicity by a LXR Inverse Agonist in a Model of Alcoholic Liver Disease. ACS Pharmacol Transl Sci 1:50-60 (2018). PubMed: 31696159