Name: Anti-Alcohol Dehydrogenase antibody [EPR4439]
Brand: RabMAb
SKU: ab108203
Rating: 5 (5 reviews)

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Primary - Rabbit Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
ICC/IF, WB, IHC-P

Protein - Recombinant Human Alcohol Dehydrogenase protein (ab123147)
SDS-PAGE, MS

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Product name

Anti-Alcohol Dehydrogenase antibody [EPR4439]
See all Alcohol Dehydrogenase primary antibodies
Description

Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Alcohol Dehydrogenase aa 1-100. The exact sequence is proprietary.
Positive control
- WB: lysate from HepG2 cells, Human fetal, mouse or rat liver; IHC-P: Human liver tissue; ICC/IF: HepG2 cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4439
Isotype

IgG
Research areas

Alternative Versions
- Anti-Alcohol Dehydrogenase antibody [EPR4439] (HRP) (ab202951)
- Anti-Alcohol Dehydrogenase antibody [EPR4439] - BSA and Azide free (ab247607)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HepG2 cytoplasmic extract lysate (ab14659)
Recombinant Protein
- Recombinant Human Alcohol Dehydrogenase protein (ab123147)

Our Abpromise guarantee covers the use of ab108203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/500 - 1/1000.
WB		1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

Application notes

Is unsuitable for Flow Cyt or IP.

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P07327 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 124 Human
- Entrez Gene: 11522 Mouse
- Entrez Gene: 24172 Rat
- Omim: 103700 Human
- SwissProt: P07327 Human
- SwissProt: P00329 Mouse
- SwissProt: P06757 Rat
- Unigene: 654433 Human
- Unigene: 40222 Rat
see all
Alternative names
- ADH alpha subunit antibody
- ADH antibody
- ADH1 antibody
- ADH1A antibody
- ADH1A_HUMAN antibody
- Alcohol dehydrogenase 1 (class I), alpha polypeptide antibody
- Alcohol dehydrogenase 1 antibody
- Alcohol dehydrogenase 1A (class I), alpha polypeptide antibody
- Alcohol dehydrogenase 1A antibody
- Alcohol dehydrogenase subunit alpha antibody
- Aldehyde reductase antibody
see all

Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Rat liver lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 40 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)This image is courtesy of an Abreview submitted by Armen Petrosyan

ab108203 staining Alcohol Dehydrogenase in the HepG2 cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50) for 1 hour at 22°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody. Nucleus was stained with DAPI (blue).
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

ab108203 at 1/250 dilution staining Alcohol Dehydrogenase in paraffin-embedded Human liver tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

ab108203 at 1/500 dilution staining Alcohol Dehydrogenase in HepG2 cells by Immunofluorescence.

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

ab108203 has not yet been referenced specifically in any publications.

Publishing research using ab108203? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mice hepatocytes)

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Loading amount

30 µg

Specification

Mice hepatocytes

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Jun 08 2018

Application

Western blot

Sample

Rat Cell lysate - whole cell (Rat hepatocytes)

Gel Running Conditions

Reduced Denaturing (4-15% SDS PAGE)

Loading amount

15 µg

Specification

Rat hepatocytes

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Apr 07 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Rat Cell (Rat hepatocytes)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Rat hepatocytes

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Apr 07 2017

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Sample

Human Cell (HEPG-2)

Specification

HEPG-2

Permeabilization

Fixative

Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Nov 04 2014

Application

Western blot

Loading amount

25 µg

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Sample

Human Cell lysate - whole cell (LNCaP)

Specification

LNCaP

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Oct 28 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

Target

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

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Western blot abreview for Anti-Alcohol Dehydrogenase antibody [EPR4439]

Western blot abreview for Anti-Alcohol Dehydrogenase antibody [EPR4439]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Alcohol Dehydrogenase antibody [EPR4439]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Alcohol Dehydrogenase antibody [EPR4439]

Western blot abreview for Anti-Alcohol Dehydrogenase antibody [EPR4439]