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    alcohol-dehydrogenase-antibody-epr4439-ab108203.pdf

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Signal Transduction Metabolism Alcohol metabolism
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RecombinantRabMAb

Recombinant Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

  • Datasheet
  • SDS
Reviews (6) Submit a question References (5)

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Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
  • Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
  • Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
  • Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

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Assay
Product image
Alcohol Dehydrogenase Assay Kit (ab102533)
Protein
Product image
Recombinant Human Alcohol Dehydrogenase protein (ab123147)
Primary
Product image
Anti-PDHA1 antibody [EPR11098] (ab168379)

View more associated products

Overview

  • Product name

    Anti-Alcohol Dehydrogenase antibody [EPR4439]
    See all Alcohol Dehydrogenase primary antibodies
  • Description

    Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: lysate from HepG2 cells, Human fetal, mouse or rat liver; IHC-P: Human liver tissue; ICC/IF: HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4439
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • HRP Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab202951)
    • Anti-Alcohol Dehydrogenase antibody [EPR4439] - BSA and Azide free (ab247607)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Alcohol Dehydrogenase protein (ab123147)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (3)
1/500 - 1/1000.
WB (3)
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Notes
ICC/IF
1/500 - 1/1000.
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Sequence similarities

    Belongs to the zinc-containing alcohol dehydrogenase family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P07327 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 124 Human
    • Entrez Gene: 11522 Mouse
    • Entrez Gene: 24172 Rat
    • Omim: 103700 Human
    • SwissProt: P07327 Human
    • SwissProt: P00329 Mouse
    • SwissProt: P06757 Rat
    • Unigene: 654433 Human
    • Alternative names

      • ADH alpha subunit antibody
      • ADH antibody
      • ADH1 antibody
      • ADH1A antibody
      • ADH1A_HUMAN antibody
      • Alcohol dehydrogenase 1 (class I), alpha polypeptide antibody
      • Alcohol dehydrogenase 1 antibody
      • Alcohol dehydrogenase 1A (class I), alpha polypeptide antibody
      • Alcohol dehydrogenase 1A antibody
      • Alcohol dehydrogenase subunit alpha antibody
      • Aldehyde reductase antibody
      see all

    Images

    • Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
      Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
      All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Mouse liver lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : Rat liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 40 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
      Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)This image is courtesy of an Abreview submitted by Armen Petrosyan

      ab108203 staining Alcohol Dehydrogenase in the HepG2 cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50) for 1 hour at 22°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody. Nucleus was stained with DAPI (blue).

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

      ab108203 at 1/250 dilution staining Alcohol Dehydrogenase in paraffin-embedded Human liver tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
      Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
      ab108203 at 1/500 dilution staining Alcohol Dehydrogenase in HepG2 cells by Immunofluorescence.
    • Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
      Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab108203? Please let us know so that we can cite the reference in this datasheet.

    ab108203 has been referenced in 5 publications.

    • Manca S  et al. The Role of Alcohol-Induced Golgi Fragmentation for Androgen Receptor Signaling in Prostate Cancer. Mol Cancer Res 17:225-237 (2019). PubMed: 30224543
    • Sonoda H  et al. The retinol-retinoic acid metabolic pathway is impaired in the lumbar spine of a rat model of congenital kyphoscoliosis. J Cell Biochem 120:15007-15017 (2019). PubMed: 31056772
    • Gao Q  et al. Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma. Cell 179:561-577.e22 (2019). PubMed: 31585088
    • De Logu F  et al. Schwann cells expressing nociceptive channel TRPA1 orchestrate ethanol-evoked neuropathic pain in mice. J Clin Invest 129:5424-5441 (2019). PubMed: 31487269
    • Sengupta M  et al. Inhibition of Hepatotoxicity by a LXR Inverse Agonist in a Model of Alcoholic Liver Disease. ACS Pharmacol Transl Sci 1:50-60 (2018). PubMed: 31696159

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    1-6 of 6 Abreviews

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Alcohol Dehydrogenase antibody [EPR4439]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Aug 03 2020

    Western blot abreview for Anti-Alcohol Dehydrogenase antibody [EPR4439]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mice hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    30 µg
    Specification
    Mice hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Jun 08 2018

    Western blot abreview for Anti-Alcohol Dehydrogenase antibody [EPR4439]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (4-15% SDS PAGE)
    Loading amount
    15 µg
    Specification
    Rat hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Apr 07 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Alcohol Dehydrogenase antibody [EPR4439]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Apr 07 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Alcohol Dehydrogenase antibody [EPR4439]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample
    Human Cell (HEPG-2)
    Specification
    HEPG-2
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Nov 04 2014

    Western blot abreview for Anti-Alcohol Dehydrogenase antibody [EPR4439]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Sample
    Human Cell lysate - whole cell (LNCaP)
    Specification
    LNCaP
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Oct 28 2014

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