Recombinant
RabMAb

Recombinant Anti-Alcohol Dehydrogenase antibody [EPR4439] (HRP) (ab202951)

Overview

  • Product name

    Anti-Alcohol Dehydrogenase antibody [EPR4439] (HRP)
    See all Alcohol Dehydrogenase primary antibodies
  • Description

    Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Alcohol Dehydrogenase aa 1-100. The exact sequence is proprietary.
    Database link: P07327

  • Positive control

    • WB: Mouse liver, Human fetal liver and Rat liver tissue lysates. IHC-P: FFPE normal human liver tissue sections.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab202951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 37,40 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/125. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • IHC image of Alcohol Dehydrogenase staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins.  The section was then incubated with ab202951, 1/125 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (HRP) (ab202951) at 1/5000 dilution

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Human) Tissue Lysate - fetal normal tissue
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 37,40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202951 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

     

References

ab202951 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab202951.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up