Product nameAnti-Alcohol Dehydrogenase antibody [EPR4439] (HRP)
See all Alcohol Dehydrogenase primary antibodies
DescriptionRabbit monoclonal [EPR4439] to Alcohol Dehydrogenase (HRP)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Alcohol Dehydrogenase aa 1-100. The exact sequence is proprietary.
Database link: P07327
- WB: Mouse liver, Human fetal liver and Rat liver tissue lysates. IHC-P: FFPE normal human liver tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab202951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 37,40 kDa (predicted molecular weight: 40 kDa).|
|IHC-P||1/125. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family.
- Information by UniProt
- ADH alpha subunit antibody
- ADH antibody
- ADH1 antibody
IHC image of Alcohol Dehydrogenase staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab202951, 1/125 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (HRP) (ab202951) at 1/5000 dilution
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Liver (Human) Tissue Lysate - fetal normal tissue
Lane 3 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 37,40 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202951 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab202951 has not yet been referenced specifically in any publications.