Western blot abreview for Anti-Alcohol Dehydrogenase antibody [EPR4440]

Average
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (white adipose tissue)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
Specification
white adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
NA
Exposure
30 second(s)
Bands
Specific: 50 kDa Non-specific: 64, 35 kDa
Positive control
NA

Additional data

PubMed ID
25703837: View

Dr. William Zhang

Verified customer

Submitted Mar 06 2018

Sign up