Overview

  • Product name

    Alcohol Dehydrogenase Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell culture media
  • Assay type

    Enzyme activity
  • Sensitivity

    = 0.01 mU/well
  • Assay time

    0h 40m
  • Product overview

    Abcam's Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
    Visit our FAQs page for tips and troubleshooting.


    Alcohol dehydrogenase assay protocol summary:
    - add samples and standards to wells
    - add reaction mix - incubate for 3 min
    - analyze with microplate reader, incubate for 30 min to 2 hrs and analyze again

  • Notes

    Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    ADH Assay Buffer WM 1 x 25ml
    ADH Positive Control (Lyophilized) Green 1 vial
    Developer (Lyophilized) Red 1 vial
    Isopropanol Blue 1 x 1ml
    NADH Standard (Lyophilized) Yellow 1 vial
  • Research areas

  • Relevance

    Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
  • Cellular localization

    Cytoplasmic
  • Alternative names

    • ADH
    • ADH alpha subunit
    • ADH beta subunit
    • ADH1
    • ADH1A
    • ADH1B
    • ADH2
    • Alcohol dehydrogenase 1A
    • Alcohol dehydrogenase 1A (class I) alpha polypeptide
    • Alcohol dehydrogenase 1B
    • Alcohol dehydrogenase 1B (class I) beta polypeptide
    • Alcohol dehydrogenase 2 (class I) beta polypeptide
    • Alcohol dehydrogenase subunit alpha
    • Alcohol dehydrogenase subunit beta
    • Aldehyde reductase
    see all

Images

  • Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

  • Sample: Bovine Liver extraction (36µg protein)

Protocols

References

This product has been referenced in:

  • Baxter M  et al. Phenotypic and functional analyses show stem cell-derived hepatocyte-like cells better mimic fetal rather than adult hepatocytes. J Hepatol 62:581-9 (2015). Read more (PubMed: 25457200) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Q&A

Answer

As long as there is active Alcohol Dehydrogenase in the plant extract, our kit ab102533 should work and you will have our guarantee
This means, as the kit doesn't have a species restriction, we cannot offer you our testing discount.

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Answer



I can confirm that this kit is tested and guaranteed for cell culture supernatant as well as serum.

Please treat the cell culture supernatant samples as describes for serum:

For cell culture supernatant samples, 5 – 50 μl serum can be directly tested. Adjust the final volume of test samples to 50 μl/well with Assay Buffer in the 96-well plate.

We suggest testing several doses of your sample to make sure the readings are within the linear range of the standard curve.

NAD(P)H or other enzymes in samples may give non-specific readings, set up the background control (see next step below) to subtract the non-specific background interference in samples.

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Answer

Nous n'avons malheureusement pas de données expérimentales ni de protocole recommandée quant à l'utilisation de kits enzymatiques avec des inhibiteurs. Je pense personnellement qu'une simple incubation de 10 minutes devrait être suffisante. Je recommanderais tout de même de vérifier cet opinion en consultant la littérature. Concernant l'offre "3 pour 2", c'est exact, 1 kit offert pour l'achat de 2 kits. Cette offre est valable jusqu'au 9 décembre 2011. J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Question
Answer

Nos collaborateurs qui produisent les kits ab83369, ab83464 et ab102533 m'ont confirmé qu'il est en effet possible d'effectuer des tests d'inhibition avec ces produits. Autre bonne nouvelle, nous avons actuellement un offre promotionnelle "3 pour 2" valable jusqu'au 9 décembre 2011 pour l'achat de kits. Le code à indiquer sur votre bon de commande est : 3FOR2-NO-T7TQW. J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Answer

This kit is compatible with all mammalian samples and hence will be compatible with rat samples as well. An example of blood sample preparation for the kit: Collect blood using citrate or EDTA. Centrifuge at 1,000 x g for 10 min at 4°C. Transfer the plasma layer to a new tube without disturbing the buffy layer and store at -80°C until ready for analysis. Remove the buffy layer from the red cell pellet. Resuspend the erythrocytes in 5x volume of ice cold distilled water and centrifuge at 10,000 x g for 10 min to pellet the erythrocyte membranes. Store the supernatant at -80°C until ready for analysis. Plasma can be diluted approx. 3-10x and the red cell lysate diluted approx. 100x prior to the assay.

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