Overview

  • Product name
    Alcohol Dehydrogenase Assay Kit
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell culture media
  • Assay type
    Enzyme activity
  • Sensitivity
    = 0.01 mU/well
  • Assay time
    0h 40m
  • Product overview

    Abcam's Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
    Visit our FAQs page for tips and troubleshooting.


    Alcohol dehydrogenase assay protocol summary:
    - add samples and standards to wells
    - add reaction mix - incubate for 3 min
    - analyze with microplate reader, incubate for 30 min to 2 hrs and analyze again

  • Notes

    Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.

  • Platform
    Microplate reader

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    ADH Assay Buffer WM 1 x 25ml
    ADH Positive Control (Lyophilized) Green 1 vial
    Developer (Lyophilized) Red 1 vial
    Isopropanol Blue 1 x 1ml
    NADH Standard (Lyophilized) Yellow 1 vial
  • Research areas
  • Relevance
    Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • ADH
    • ADH alpha subunit
    • ADH beta subunit
    • ADH1
    • ADH1A
    • ADH1B
    • ADH2
    • Alcohol dehydrogenase 1A
    • Alcohol dehydrogenase 1A (class I) alpha polypeptide
    • Alcohol dehydrogenase 1B
    • Alcohol dehydrogenase 1B (class I) beta polypeptide
    • Alcohol dehydrogenase 2 (class I) beta polypeptide
    • Alcohol dehydrogenase subunit alpha
    • Alcohol dehydrogenase subunit beta
    • Aldehyde reductase
    see all

Images

  • Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

  • Sample: Bovine Liver extraction (36µg protein)

Protocols

References

This product has been referenced in:
  • Baxter M  et al. Phenotypic and functional analyses show stem cell-derived hepatocyte-like cells better mimic fetal rather than adult hepatocytes. J Hepatol 62:581-9 (2015). Read more (PubMed: 25457200) »
See 1 Publication for this product

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Submitted Jun 05 2018

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