Product nameAlcohol dehydrogenase Assay Kit
See all Alcohol Dehydrogenase kits
Sample typeCell Lysate, Tissue Lysate
Range0.4 mU/ml - 80 mU/ml
Alcohol Dehydrogenase Assay Kit (ab272518) is a non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Fast and Sensitive: Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Assay Buffer 1 x 10ml Calibrator 1 x 1.5ml Diaphorase 1 x 120µl MTT Solution 1 x 1.5ml NAD Solution 1 x 1ml Substrate (10% Ethanol) 1 x 1ml
RelevanceAlcohol dehydrogenase (Alcohol DH, ADH) (EC 220.127.116.11) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
- ADH alpha subunit
- ADH beta subunit
Our Abpromise guarantee covers the use of ab272518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab272518 has not yet been referenced specifically in any publications.