Overview

  • Product name

    Aldehyde Assay Kit (Fluorometric)
    See all Aldehyde kits
  • Detection method

    Fluorescent
  • Sample type

    Saliva, Urine, Plasma, Cell Lysate, Cell culture media
  • Assay type

    Quantitative
  • Sensitivity

    3 µM
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Abcam's Aldehyde Quantification Assay Kit (Fluorometric) (ab138882) is used for quantifying aldehydes at higher pH by using a proprietary fluorogenic dye that generates a strongly fluorescent product upon reacting with an aldehyde.  This fluorimetric kit provides a sensitive mix-and-read method to detect as little as 0.3 nanomole of aldehyde/100 µL assay volume (3 µM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be read by a fluorescence microplate reader at Ex/Em = 365/435 nm.

  • Notes

    The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years. Rapid and accurate measurement of aldehydes is an important task for biological research, chemical research, food industry and environmental pollution surveillance. There are a few reagents or assay kits available for quantifying the number of aldehydes. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS.

  • Platform

    Microplate reader

Properties

Images

  • Aldehyde levels measured in cell supernatants (μM). Samples were diluted 2 fold.

  • Aldehyde levels measured in cell lysates showing quantity (µmol) per 1 mln cells.

    Samples with the concentration of 1e8 cells/mL (HepG2) and 1.28e8 cells/mL (HeLa) were used. Samples were diluted 100 fold.

  • Aldehyde levels measured in biological fluids (μM). Samples were diluted 1-10 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Aldehyde dose response was measured in a solid black 96-well plate with ab138882 using a fluorescence microplate reader. As low as 3 µM of aldehyde can be detected with 15 minutes incubation (n=3).

Protocols

References

This product has been referenced in:

  • Suh HW  et al. Biodegradable bioadhesive nanoparticle incorporation of broad-spectrum organic sunscreen agents. Bioeng Transl Med 4:129-140 (2019). Read more (PubMed: 30680324) »
  • Deng Y  et al. Improved i.p. drug delivery with bioadhesive nanoparticles. Proc Natl Acad Sci U S A 113:11453-11458 (2016). Read more (PubMed: 27663731) »
See all 4 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

The standard contains glyceraldhehyde.

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Answer

Thank you for your inquiry.

I have heard back from the supplying lab that yes, the set up of your reader should be fine.

I hope this information helps. Please contact us with any other questions.

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Answer

I have confirmed with the testing laboratory that the kit is compatible with your plate reader using Ex/Em 350/460 nm.

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Question
Answer

Vielen Dank für Ihren Anruf.

Ich habe gute Nachrichten für Sie. Hier die Antwort aus unserem Labor:

"The dye is non-polar. The buffer pH is 7.5 for assay buffer, and 12 for reaction buffer. Hope it helps. Thanks!"

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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