Overview

  • Product name

    Aldehyde Assay Kit II (Colorimetric - Blue)
    See all Aldehyde kits
  • Detection method

    Colorimetric
  • Sample type

    Other biological fluids, Cell Lysate
  • Assay type

    Quantitative
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Aldehyde Assay Kit II (Colorimetric - Blue) (ab219923) provides a sensitive mix-and-read method to detect aldehyde in a variety of samples such as cell lysates or biological fluids. This assay used a proprietary sensor that upon reacting with an aldehyde, generates a chromogenic product that can be detected by absorbance on a microplate reader at OD620-660 nm. This assay can detect as little as 3 µM aldehyde (0.3 nmol/100 µL assay volume).


    The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation step.


    If your plate reader does not have the appropriate filter to detect the absorbance change, we recommend Aldehyde Quantification Assay Kit (Colorimetric) (ab112113), which can be read at OD405 or OD550 nm.

  • Notes

    Very reactive aldehydes, namely 4-hydroxyalkenals, were first shown to be formed in autoxidizing chemical systems. It was subsequently shown that 4-hydroxyalkenals, particularly 4-hydroxynonenal, were formed in substantial amounts under biological conditions, i.e. during the peroxidation of lipids of liver microsomes incubated in the NADPH-Fe system. Many other aldehydes were also identified in peroxidizing liver microsomes orhepatocytes, e.g., alkanals, alk-2-enals, and 4-hydroxyalkenals.

    Rapid and accurate measurement of aldehydes is an important task for biological and chemical research, as well as food industry and environmental pollution surveillance.

  • Platform

    Microplate reader

Properties

Images

  • Aldehyde Quantification Assay Kit II (Colorimetric) (ab219923). Typical aldehyde standard calibration curve. Aldehyde dose response was measured in a white wall/clear bottom 96-well plate using a SpectraMax microplate reader. As low as 3 µM aldehyde can be detected with 30 minutes incubation (n=3).

Protocols

References

ab219923 has not yet been referenced specifically in any publications.

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