Recombinant
RabMAb

Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171)

Overview

  • Product name
    Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)]
    See all Aldehyde dehydrogenase 10 primary antibodies
  • Description
    Rabbit monoclonal [EPR15425(B)] to Aldehyde dehydrogenase 10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Aldehyde dehydrogenase 10 aa 300-400. The exact sequence is proprietary.
    Database link: P51648

  • Positive control
    • A549, 293T and U87-MG cell lysates; A549 and HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF 1/250.
Flow Cyt 1/170.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length.
  • Involvement in disease
    Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS) [MIM:270200]. SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects.
  • Sequence similarities
    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ahd 3 antibody
    • Ahd 3r antibody
    • Ahd3 antibody
    • Ahd3 r antibody
    • AL3A2_HUMAN antibody
    • Aldehyde dehydrogenase 10 antibody
    • Aldehyde dehydrogenase 3 antibody
    • Aldehyde dehydrogenase 3 family, member A2 antibody
    • Aldehyde dehydrogenase family 3 member A2 antibody
    • Aldehyde dehydrogenase family 3, subfamily A2 antibody
    • Aldehyde dehydrogenase, family 3, subfamily A, member 2 antibody
    • ALDH10 antibody
    • Aldh3 antibody
    • ALDH3A2 antibody
    • Aldh4 antibody
    • Aldh4 r antibody
    • Aldh4r antibody
    • DKFZp686E23276 antibody
    • FALDH antibody
    • Fatty aldehyde dehydrogenase antibody
    • FLJ20851 antibody
    • Microsomal aldehyde dehydrogenase antibody
    • msALDH antibody
    • SLS antibody
    see all

Images

  • All lanes : Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/2000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

  • Immunofluorescence analysis of 4% paraformaldehyde fixed A549 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/250 dilution (red). A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue).

  • Flow cytometric analysis of 2% paraformaldehyde fixed HepG2 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/170 dilution (red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Isotype control: Rabbit monoclonal IgG (green).

  • Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/10000 dilution + U87-MG cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

References

ab184171 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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