Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
10 µg
Treatment
RNAi control or ALDH3A2, 72h
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS/0.01% Tween-20
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: HRP AffiniPure Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
HeLa cell lysate treated with ALDH3A2 siRNA for 72h
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 54 kDa Non-specific: 62 kDa
Positive control
HeLa cell lysate treated with control siRNA for 72h
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Submitted Oct 21 2019