Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue Culture Media
- Sensitivity: 0.05 mU/well
Product nameALDH Activity Assay Kit (Fluorometric)
See all Aldehyde Dehydrogenase kits
Sample typeCell culture supernatant, Serum, Plasma, Cell Lysate, Tissue Culture Media
Assay typeEnzyme activity (quantitative)
Sensitivity< 0.05 mU/well
Species reactivityReacts with: Mammals, Other species
Abcam's PicoProbe ALDH Activity Assay Kit (ab155894) is a robust tool to quantify ALDH (aldehyde dehydrogenase) enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH to form the NADH which couples with the PicoProbe to generate a potent fluorescence (Ex/Em = 535/587). The ALDH fluorometric assay kit is 10 times more sensitive than the ALDH colorimetric assay and can detect < 0.05 mU ALDH activity (based on our unit definition) in a variety of samples.
The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Acetaldehyde Purple 1 x 0.5ml ALDH Assay Buffer WM 1 x 25ml ALDH Positive Control Green 1 vial ALDH Substrate Mix Red 1 vial NADH Standard Yellow 1 vial PicoProbe Blue 1 x 0.4ml
ab155894 has not yet been referenced specifically in any publications.