Anti-ALDH1A1 antibody [5A11] (ab105920)

Submit a review Q&A (2)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [5A11] (ab105920)
  • Flow Cytometry - Anti-ALDH1A1 antibody [5A11] (ab105920)
  • Western blot - Anti-ALDH1A1 antibody [5A11] (ab105920)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [5A11] (ab105920)

Key features and details

  • Mouse monoclonal [5A11] to ALDH1A1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-ALDH1A1 antibody [5A11]
    See all ALDH1A1 primary antibodies

  • Description

    Mouse monoclonal [5A11] to ALDH1A1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Purified recombinant fragment of Human ALDH1A1 expressed in E. coli.

  • Positive control

    • WB: Raji, Jurkat, THP1 and K562 cell lysates IHC-P: Human cerebellum and lung cancer tissues

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR120645-17 are from Tissue Culture Supernatant

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab105920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.

  • Pathway

    Cofactor metabolism; retinol metabolism.

  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Acetaldehyde dehydrogenase 1 antibody
      • AHD2 antibody
      • AL1A1_HUMAN antibody
      • ALDC antibody
      • Aldehyde dehydrogenase 1 family member A1 antibody
      • Aldehyde dehydrogenase 1 soluble antibody
      • Aldehyde dehydrogenase 1A1 antibody
      • Aldehyde dehydrogenase antibody
      • Aldehyde dehydrogenase cytosolic antibody
      • Aldehyde dehydrogenase family 1 member A1 antibody
      • Aldehyde dehydrogenase liver cytosolic antibody
      • ALDH 1 antibody
      • ALDH 1A1 antibody
      • ALDH class 1 antibody
      • ALDH, liver cytosolic antibody
      • ALDH-E1 antibody
      • ALDH1 A1 antibody
      • ALDH1 antibody
      • ALDH11 antibody
      • ALDH1A1 antibody
      • ALHDII antibody
      • cytosolic antibody
      • epididymis luminal protein 12 antibody
      • epididymis luminal protein 9 antibody
      • epididymis secretory sperm binding protein Li 53e antibody
      • HEL-S-53e antibody
      • MGC2318 antibody
      • PUMB1 antibody
      • RALDH 1 antibody
      • RalDH1 antibody
      • Retinal dehydrogenase 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [5A11] (ab105920)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [5A11] (ab105920)
      ab105920, at a 1/200 dilution, staining ALDH1A1 in paraffin embedded Human cerebellum tissue by Immunohistochemistry.
    • Flow Cytometry - Anti-ALDH1A1 antibody [5A11] (ab105920)
      Flow Cytometry - Anti-ALDH1A1 antibody [5A11] (ab105920)
      Overlay histogram showing HepG2 cells stained with ab105920 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105920, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Western blot - Anti-ALDH1A1 antibody [5A11] (ab105920)
      Western blot - Anti-ALDH1A1 antibody [5A11] (ab105920)
      All lanes : Anti-ALDH1A1 antibody [5A11] (ab105920) at 1/200 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : THP1 cell lysate
      Lane 4 : K562 cell lysate

      Predicted band size: 55 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [5A11] (ab105920)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [5A11] (ab105920)
      ab105920, at a 1/200 dilution, staining ALDH1A1 in paraffin embedded Human lung cancer tissue by Immunohistochemistry.

    References (5)

    Publishing research using ab105920? Please let us know so that we can cite the reference in this datasheet.

    ab105920 has been referenced in 5 publications.

    • Gunduz M  et al. Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer. Oncol Lett 19:965-971 (2020). PubMed: 31897209
    • Sulaiman A  et al. Dual inhibition of Wnt and Yes-associated protein signaling retards the growth of triple-negative breast cancer in both mesenchymal and epithelial states. Mol Oncol N/A:N/A (2018). WB . PubMed: 29316250
    • Jia D  et al. An autocrine inflammatory forward-feedback loop after chemotherapy withdrawal facilitates the repopulation of drug-resistant breast cancer cells. Cell Death Dis 8:e2932 (2017). PubMed: 28703802
    • Jia D  et al. Cardamonin reduces chemotherapy-enriched breast cancer stem-like cells in vitro and in vivo. Oncotarget 7:771-85 (2016). WB . PubMed: 26506421
    • Martinez-Cruzado L  et al. Aldh1 Expression and Activity Increase During Tumor Evolution in Sarcoma Cancer Stem Cell Populations. Sci Rep 6:27878 (2016). WB, IF . PubMed: 27292183

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Some question regarding the positive control for abcam antibodies. I would like to enquire about positive control for IHC for the 3 monoclonal antibody as following: Cat No Product Name Suggested Positive control QUESTION M7802 Mo a Hu Cd44 -Can I use normal human spleen/thymus/tonsil? Ab3380 Anti-BCRP/ABCG2 antibody Caco Cell lysate Any other alternative? I don’t have this. Ab105920 Anti-ALDH1A1 THP1 and K562 cell lysate, human cerebellum and lung cancer tissue Can I use normal human liver? Hope to receive your soonest reply. Thanks Best regards,

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    Answer

    Thank you for contacting us. ab3380; The ABCG2 target is expressed in tonsils, spleen, gall bladder and lungs etc so the sections of these tissues will be good positive control. You can find the complete expression information on Human Protein Atlas website; http://www.proteinatlas.org/ENSG00000118777/normal ab4372; ab87930 are spleen tissue slides ab4349 is human lung tissue slide ab105920; normal liver sections can be used; this target is expressed in normal liver. http://www.proteinatlas.org/ENSG00000165092/normal ab4348 is normal liver tissue slide. M7802; this does not seems to be Abcam product however CD44 is highly expressed in spleen, tonsils etc so the tissue sections of these will be a good positive control. http://www.proteinatlas.org/ENSG00000026508/normal I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Check the Abcam range of IHC kits also; https://www.abcam.com/index.html?pageconfig=resource&rid=12906 https://www.abcam.com/index.html?pageconfig=resource&rid=12853 https://www.abcam.com/index.html?pageconfig=resource&rid=12886

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    Question

    want antibody that will NOT cross react with mouse

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    Answer

    Thank you for contacting us. Unfortunately we do not have an antibody that suits your needs. All of the ALDH1A1 antibodies have either been tested in mouse or have sufficient homology (greater than 80%) with the immunogen so that they are likely to react. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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